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anti-Hamster Insulin antibody for Immunohistochemistry (Frozen Sections)

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Reactivity
Hamster, Human, Pig (Porcine), Rat (Rattus)
899
376
252
243
241
119
49
5
5
4
4
4
3
2
2
1
1
1
1
1
1
Host
Guinea Pig
812
180
56
7
2
2
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
55
41
40
27
25
24
19
19
17
17
17
17
17
17
17
17
16
16
12
10
7
7
5
5
5
5
5
2
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
582
345
234
203
187
180
150
128
85
56
53
28
13
12
9
8
7
6
5
5
4
3
2
1
1
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Catalog No. ABIN115757
$ 797.50
Plus shipping costs $45.00

Top referenced anti-Insulin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Insulin Antibodies

Application / Reactivity Human Pig (Porcine) Rat (Rattus) Hamster
Western Blotting (WB) 212 Antibodies 108 Antibodies 44 Antibodies
Radioimmunoassay (RIA) 46 Antibodies 14 Antibodies 4 Antibodies
Protein Assay (PrA) 6 Antibodies 2 Antibodies
Immunoradiometric Assay (IRMA) 1 Antibodies
Immunoprecipitation (IP) 85 Antibodies 55 Antibodies 27 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 323 Antibodies 200 Antibodies 102 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 185 Antibodies

General

Antigen Insulin (INS) Antibodies
Reactivity Hamster, Human, Pig (Porcine), Rat (Rattus)
(899), (376), (252), (243), (241), (119), (49), (5), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Guinea Pig
(812), (180), (56), (7), (2), (2)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(55), (41), (40), (27), (25), (24), (19), (19), (17), (17), (17), (17), (17), (17), (17), (17), (16), (16), (12), (10), (7), (7), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(582), (345), (234), (203), (187), (180), (150), (128), (85), (56), (53), (28), (13), (12), (9), (8), (7), (6), (5), (5), (4), (3), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN115757) Images
Specificity The antibody recognizes Insulin produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors. Absorption with 10-100 μg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and Insulin only partly reduce staining.
Cross-Reactivity (Details) Also reacts to: Proinsulin
Purification Serum
Immunogen Synthetic Human proinsulin

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin Antibody (ABIN115757) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin Antibody (ABIN115757) Images back to top
Application Notes Immunofluorescence: 1: 1000 - 1: 2000, overnight incubation at 2-8C. Immunohistochemistry on frozen and paraffin sections: 1: 3000 - 1: 5000. Positive Control: Formalin-fixed paraffin sections of human pancreas
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin Antibody (ABIN115757) Images back to top
Format Liquid
Reconstitution Dissolve the antiserum in 50-100 μL distilled water, and dilute further in 0.1 M PBS with 1 % BSA and 0.09 % Sodium Azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.

References for anti-Insulin Antibody (ABIN115757)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Kohnert, Axcrona, Hehmke, Klöting, Sundler, Ahrén: "Islet neuronal abnormalities associated with impaired insulin secretion in type 2 diabetes in the Chinese hamster." in: Regulatory peptides, Vol. 82, Issue 1-3, pp. 71-9, 1999

Ahrén, Gudbjartsson, Al-Amin, Mårtensson, Myrsén-Axcrona, Karlsson, Mulder, Sundler: "Islet perturbations in rats fed a high-fat diet." in: Pancreas, Vol. 18, Issue 1, pp. 75-83, 1999

Myrsén-Axcrona, Karlsson, Sundler, Ahrén: "Dexamethasone induces neuropeptide Y (NPY) expression and impairs insulin release in the insulin-producing cell line RINm5F. Release of NPY and insulin through different pathways." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10790-6, 1997

Myrsén, Ahrén, Sundler: "Dexamethasone-induced neuropeptide Y expression in rat islet endocrine cells. Rapid reversibility and partial prevention by insulin." in: Diabetes, Vol. 45, Issue 10, pp. 1306-16, 1996