anti-Human Insulin antibody for Immunofluorescence

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Human, Mouse (Murine), Rat (Rattus)
941
399
283
273
262
121
49
6
6
5
5
5
4
3
2
1
1
1
1
1
1
1
Host
Rabbit
830
178
55
16
3
1
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
55
42
41
27
26
26
21
21
18
17
17
17
17
17
17
16
16
12
11
10
9
9
9
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
617
373
244
228
207
203
160
138
69
66
38
28
14
11
9
9
8
7
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN372838
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN113470 IHC (fro) IF IHC (p) Guinea Pig Log in to see Polyclonal 2
1 ABIN356469 IHC (fro) IF IHC (p) Rabbit IgG Log in to see Polyclonal 1
1 ABIN617877 IEM ICC IF IHC Rabbit IgG Log in to see Polyclonal 44
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139
1 ABIN269045 ICC IF IHC (p) IHC RIA ELISA Mouse IgG1 Log in to see C-PEP-01
1 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10
1 ABIN3026543 FACS IF IHC Mouse IgG1, kappa AA 1-84 Log in to see 2D11-H5 or INS05
1 ABIN115757 IHC (fro) IF IHC (p) Guinea Pig Log in to see Polyclonal 4
1 ABIN3026546 FACS IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04
1 ABIN3026326 FACS IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05
1 ABIN337249 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN2294343 FACS IF IP IHC ELISA WB Mouse IgG1 kappa AA 1-84 Log in to see 2D11-H5
1 ABIN4326145 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4326146 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4326016 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326144 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4326112 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326028 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326029 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139

Top referenced anti-Insulin antibody for Immunofluorescence

Similar anti-Insulin Antibodies

Application / Reactivity Rat (Rattus)
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 126 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 56 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 38 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 66 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 112 Antibodies
Immunoprecipitation (IP) 26 Antibodies

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(941), (399), (283), (273), (262), (121), (49), (6), (6), (5), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(830), (178), (55), (16), (3), (1)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(55), (42), (41), (27), (26), (26), (21), (21), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(617), (373), (244), (228), (207), (203), (160), (138), (69), (66), (38), (28), (14), (11), (9), (9), (8), (7), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) Images
Specificity This antibody recognizes endogenous levels of total Human Insulin protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Immunogen INS antibody was raised against Synthetic peptide (KLH-coupled) derived from the sequence of Human Insulin.
Isotype IgG

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) Images back to top
Application Notes ELISA. Flow Cytometry. Immunofluorescence. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details ProductDetails: References for anti-Insulin antibody (ABIN372838) Images back to top
Format Liquid
Concentration 0.02 mg/mL
Buffer 10 mM HEPES, pH 7.5, 150 mM Sodium Chloride, 100 μg/mL BSA, 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-Insulin antibody (ABIN372838)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Micucci, Orciari, Catalano: "Hyperglycemia promotes K-Ras-induced lung tumorigenesis through BASCs amplification." in: PLoS ONE, Vol. 9, Issue 8, pp. e105550, 2014

Madsen, Knauf, Gotfredsen, Pilling, Sjögren, Andersen, Andersen, de Boer, Manova, Barlas, Vundavalli, Nyborg, Knudsen, Moelck, Fagin: "GLP-1 receptor agonists and the thyroid: C-cell effects in mice are mediated via the GLP-1 receptor and not associated with RET activation." in: Endocrinology, Vol. 153, Issue 3, pp. 1538-47, 2012

"Retraction." in: Endocrinology, 2010

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) back to top
Supplier Images
 image for anti-Insulin antibody (INS) (ABIN372838) anti-Insulin (INS) antibody