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anti-Human Insulin antibody for Immunoprecipitation

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Reactivity
Human, Pig (Porcine)
886
368
249
238
235
118
48
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Host
Mouse
796
180
57
7
2
2
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
55
41
40
27
25
24
19
19
17
17
17
17
17
17
17
17
16
16
12
10
7
7
5
5
5
5
5
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
570
341
233
196
182
180
150
127
84
56
52
26
13
12
9
8
7
6
5
5
4
3
1
1
1
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Catalog No. ABIN117970
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008957968 ABIN1107760 IHC (p) IP Mouse IgG1 Log in to see ISL-8J 1
0.0008957968 ABIN129507 IP IHC ELISA WB Mouse IgG Log in to see 2D11-H5
0.0008957968 ABIN2294343 FACS IF IP IHC ELISA WB Mouse IgG1 kappa AA 1-84 Log in to see 2D11-H5
0.0008957968 ABIN1502607 IP Mouse IgG1 Log in to see ISL-8J
0.0008957968 ABIN1502608 IP Mouse IgG1 Log in to see ISL-8J
0.0008957968 ABIN2294171 FACS IF IP IHC WB Mouse IgG1 kappa AA 1-84 Log in to see E2-E3-same as INS04
0.0008957968 ABIN2294177 FACS IF IP IHC WB Mouse IgG1 kappa AA 1-84 Log in to see E2-E3-2D11-H5-same as INS04-INS05
0.0008957968 ABIN4326028 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326029 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326030 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326101 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326102 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326103 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326104 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326105 FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326106 FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326107 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326108 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326109 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM139
0.0008957968 ABIN4326111 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM139

Top referenced anti-Insulin antibody for Immunoprecipitation

Similar anti-Insulin Antibodies

Application / Reactivity Pig (Porcine)
Dot Blot (DB) 2 Antibodies
ELISA 148 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 117 Antibodies
Functional Studies (Func) 13 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 73 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 121 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 199 Antibodies
Immunoprecipitation (IP) 55 Antibodies

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Pig (Porcine)
(886), (368), (249), (238), (235), (118), (48), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(796), (180), (57), (7), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(55), (41), (40), (27), (25), (24), (19), (19), (17), (17), (17), (17), (17), (17), (17), (17), (16), (16), (12), (10), (7), (7), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(570), (341), (233), (196), (182), (180), (150), (127), (84), (56), (52), (26), (13), (12), (9), (8), (7), (6), (5), (5), (4), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN117970) Images
Sequence Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
Specificity This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
Purification Protein A Chromatography
Immunogen Purified human Insulin coupled to Bovine Serum Albumin (BSA).
Clone 2D11-H5
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin Antibody (ABIN117970) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
Molecular Weight 6 kDa
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin Antibody (ABIN117970) Images back to top
Application Notes This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10 μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imately 5 ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin Antibody (ABIN117970) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

References for anti-Insulin Antibody (ABIN117970)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Hopkins, Williams: "Insulin receptors are widely distributed in human brain and bind human and porcine insulin with equal affinity." in: Diabetic medicine : a journal of the British Diabetic Association, Vol. 14, Issue 12, pp. 1044-50, 1998

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN117970) back to top
Supplier Images
 image for anti-Insulin antibody (INS) (ABIN117970) ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Eac...
 image for anti-Insulin antibody (INS) (ABIN117970) Dot blotting. Mab anti-Insulin antibody (clone 2D11.H5) is shown to detect human insu...
 image for anti-Insulin antibody (INS) (ABIN117970) Diagram of Insulin Structure. Insulin is a polypeptide hormone produced by cells in t...