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anti-Human Insulin antibody for Radioimmunoassay

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Reactivity
Human
562
135
134
97
78
25
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Host
Mouse
446
177
57
2
2
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
37
28
25
12
10
10
6
5
5
5
5
5
5
5
5
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
385
181
139
124
67
56
49
42
38
36
25
13
12
12
9
8
7
6
5
5
4
3
1
1
1
Supplier
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Catalog No. ABIN111475
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676636 ABIN111965 EIA Func IHC (p) RIA Mouse IgG1 Log in to see IN-05 1
0.0008676636 ABIN111476 EIA RIA Mouse IgG1 Log in to see C-PEP-01 1
0.0008676636 ABIN125983 EIA Func IHC (fro) IHC (p) RIA Mouse IgG1 Log in to see IN-05 1
0.0008676636 ABIN94408 Func ICC IHC (p) RIA ELISA Mouse IgG1 Log in to see IN-05 1
0.0008676636 ABIN125702 Func ICC IHC (p) RIA ELISA Mouse IgG1 Log in to see IN-05 1
0.0008676636 ABIN235690 IHC (f) PrA RIA ELISA Mouse IgG1 Log in to see D4B8
0.0008676636 ABIN236164 IHC PrA RIA ELISA Mouse IgG1 Log in to see 3A1
0.0008676636 ABIN235687 PrA RIA ELISA Mouse IgG1 Log in to see 8-00E-02
0.0008676636 ABIN599768 IHC (p) RIA ELISA Mouse IgG1 Log in to see
0.0008676636 ABIN1839876 IF IHC (p) RIA Mouse IgG1 Log in to see K36aC10
0.0008676636 ABIN235688 PrA RIA ELISA Mouse IgG1 Log in to see 3A6
0.0008676636 ABIN235689 PrA RIA ELISA Mouse IgG1 Log in to see 7F8
0.0008676636 ABIN190133 IHC RIA ELISA Mouse IgG1 Log in to see
0.0008676636 ABIN191769 EIA IHC (fro) IF IHC (p) RIA WB Rabbit AA 57-87 Log in to see Polyclonal
0.0008676636 ABIN190132 IHC RIA ELISA Mouse IgG1 Log in to see
0.0008676636 ABIN190131 RIA ELISA Mouse IgG1 Log in to see
0.0008676636 ABIN1826074 RIA ELISA Mouse IgG1 Log in to see 423631
0.0008676636 ABIN799958 IHC RIA ELISA Mouse IgG1 Log in to see
0.0008676636 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10
0.0008676636 ABIN269045 ICC IF IHC (p) IHC RIA ELISA Mouse IgG1 Log in to see C-PEP-01

Top referenced anti-Insulin antibody for Radioimmunoassay

Similar anti-Insulin Antibodies

Application / Reactivity Human
Dot Blot (DB) 1 Antibodies
ELISA 301 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 26 Antibodies
Flow Cytometry (FACS) 39 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 8 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 47 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 168 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 48 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 119 Antibodies

General

Antigen Insulin (INS) Antibodies
Reactivity Human
(562), (135), (134), (97), (78), (25), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(446), (177), (57), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(37), (28), (25), (12), (10), (10), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
(385), (181), (139), (124), (67), (56), (49), (42), (38), (36), (25), (13), (12), (12), (9), (8), (7), (6), (5), (5), (4), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN111475) Images
Specificity This antibody clone C-PEP-01 reacts specifically with C-peptide of Proinsulin. No cross-reactivity with Insulin or other peptide hormones or proteins was observed.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Also reacts to: Proinsulin
Purification Precipitation Methods.
Immunogen Human C-peptide conjugated to Bovine Serum Albumin
Clone C-PEP-01
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin Antibody (ABIN111475) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains, later become linked together to form the Insulin molecule). From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin Antibody (ABIN111475) Images back to top
Application Notes ELISA. RIA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections (2-5 μg/mL). Pretreatment of paraffin sectionswith 0.1 % trypsin in PBS for 30 min at 37 °C is recommended. Positive Control: Human pancreas (islets of Langerhans)According to customer information this antibody does not work in Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin Antibody (ABIN111475) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-Insulin Antibody (ABIN111475)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991