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|Antigen||Insulin (INS) Antibodies|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN111475) Images
|Specificity||This antibody clone C-PEP-01 reacts specifically with C-peptide of Proinsulin. No cross-reactivity with Insulin or other peptide hormones or proteins was observed.|
Species reactivity (tested):Human.
Also reacts to: Proinsulin
|Immunogen||Human C-peptide conjugated to Bovine Serum Albumin|
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN111475) Images back to top
|Alternative Name||Insulin (INS Antibody Abstract)|
|Background||Proinsulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains, later become linked together to form the Insulin molecule). From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.Synonyms: INS|
|Research Area||Neurology, Diabetes, Hormones|
|Pathways||NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN111475) Images back to top
ELISA. RIA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections (2-5 μg/mL). Pretreatment of paraffin sectionswith 0.1 % trypsin in PBS for 30 min at 37 °C is recommended. Positive Control: Human pancreas (islets of Langerhans)According to customer information this antibody does not work in Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN111475) Images back to top
|Buffer||PBS, pH ~7.4, 15 mM Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|
References for anti-Insulin antibody (ABIN111475)Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991