anti-Human Insulin antibody for Radioimmunoassay

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Human
965
397
302
283
262
121
49
6
6
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
842
183
54
25
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
57
42
41
28
28
27
23
23
18
17
17
17
17
17
17
16
16
12
11
11
11
11
11
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
622
371
248
234
208
204
161
142
71
66
39
27
18
11
9
8
8
7
5
4
3
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN111475
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN111476 EIA RIA Mouse IgG1 Log in to see C-PEP-01 1
1 ABIN111965 EIA Func IHC (p) RIA Mouse IgG1 Log in to see IN-05 1
1 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10
1 ABIN269045 ICC IF IHC (p) IHC RIA ELISA Mouse IgG1 Log in to see C-PEP-01
1 ABIN236164 IHC PrA RIA ELISA Mouse IgG1 Log in to see 3A1
1 ABIN235687 PrA RIA ELISA Mouse IgG1 Log in to see 8-00E-02
1 ABIN235690 IHC (f) PrA RIA ELISA Mouse IgG1 Log in to see D4B8
1 ABIN94408 Func ICC IHC (p) RIA ELISA Mouse IgG1 Log in to see IN-05 1
1 ABIN206973 BR ELISA Func ICC IHC (p) Neut RIA Mouse IgG1 Log in to see IN-05
1 ABIN191769 EIA IHC (fro) IF IHC (p) RIA WB Rabbit AA 57-87 Log in to see Polyclonal
1 ABIN206900 BR ELISA Func ICC IHC (p) Neut RIA Mouse IgG1 Log in to see IN-05
1 ABIN1839876 IF IHC (p) RIA Mouse IgG1 Log in to see K36aC10
1 ABIN235688 PrA RIA ELISA Mouse IgG1 Log in to see 3A6
1 ABIN235689 PrA RIA ELISA Mouse IgG1 Log in to see 7F8
1 ABIN3200648 ELISA Func ICC IHC (p) RIA Mouse IgG1 Log in to see IN-05
1 ABIN190131 RIA ELISA Mouse IgG1 Log in to see
1 ABIN190132 IHC RIA ELISA Mouse IgG1 Log in to see
1 ABIN190133 IHC RIA ELISA Mouse IgG1 Log in to see
1 ABIN4347579 ELISA ICC IF IHC IHC (p) RIA PerCP Mouse IgG1 Log in to see C-PEP-01
1 ABIN3200649 ELISA Func ICC IHC (p) RIA Mouse IgG1 Log in to see IN-05

General

Antigen Insulin (INS) Antibodies
Reactivity Human
(965), (397), (302), (283), (262), (121), (49), (6), (6), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(842), (183), (54), (25), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(57), (42), (41), (28), (28), (27), (23), (23), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (11), (11), (11), (11), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
(622), (371), (248), (234), (208), (204), (161), (142), (71), (66), (39), (27), (18), (11), (9), (8), (8), (7), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin antibody (ABIN111475) Images
Specificity This antibody clone C-PEP-01 reacts specifically with C-peptide of Proinsulin. No cross-reactivity with Insulin or other peptide hormones or proteins was observed.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Also reacts to: Proinsulin
Purification Precipitation Methods.
Immunogen Human C-peptide conjugated to Bovine Serum Albumin
Clone C-PEP-01
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin antibody (ABIN111475) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains, later become linked together to form the Insulin molecule). From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin antibody (ABIN111475) Images back to top
Application Notes ELISA. RIA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections (2-5 μg/mL). Pretreatment of paraffin sectionswith 0.1 % trypsin in PBS for 30 min at 37 °C is recommended. Positive Control: Human pancreas (islets of Langerhans)According to customer information this antibody does not work in Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin antibody (ABIN111475) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-Insulin antibody (ABIN111475)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Background publications

Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991