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anti-Human Insulin antibody for Western Blotting

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Reactivity
Cow (Bovine), Human, Pig (Porcine)
897
376
253
246
240
119
49
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
812
178
57
7
2
2
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
55
41
40
27
25
24
19
19
17
17
17
17
17
17
17
17
16
16
12
10
7
7
5
5
5
5
5
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
582
345
231
203
187
178
149
128
84
56
52
27
12
12
9
8
7
6
5
5
4
3
2
1
1
Supplier
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Catalog No. ABIN487416
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12633176 ABIN411521 WB Rabbit IgG Log in to see Polyclonal
0.12633176 ABIN411565 IHC ELISA WB Mouse IgG1 full length Log in to see HPI-B5
0.12633176 ABIN2706403 WB Rabbit Center Log in to see Polyclonal
0.12633176 ABIN2706402 WB Rabbit Center Log in to see Polyclonal
0.12633176 ABIN1873240 IHC WB Rabbit IgG Log in to see Polyclonal
0.12633176 ABIN729118 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.12633176 ABIN1539839 WB Mouse IgM AA 35-64 Log in to see 396CT20-4-4
0.12633176 ABIN1539840 WB Mouse IgM AA 35-64 Log in to see 396CT20-4-4
0.12633176 ABIN3020884 WB Rabbit IgG Log in to see Polyclonal
0.12633176 ABIN3023559 WB Rabbit IgG pTyr1189 Log in to see Polyclonal
0.12633176 ABIN3023553 WB Rabbit IgG pTyr1361 Log in to see
0.12633176 ABIN3022885 IHC WB Rabbit IgG Log in to see Polyclonal
0.12633176 ABIN4326017 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
0.12633176 ABIN2488138 WB Mouse IgM Log in to see 396CT20-4-4
0.12633176 ABIN2488139 WB Mouse IgM Log in to see 396CT20-4-4
0.12633176 ABIN4226797 WB Rabbit IgG Log in to see Polyclonal
0.12633176 ABIN2405054 IHC WB Rabbit IgG Log in to see Polyclonal
0.001181628 ABIN117970 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 2D11-H5 1
0.001181628 ABIN272321 EIA IHC (p) WB Mouse IgG1 Log in to see E2E3 3
0.001181628 ABIN1584615 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 1

Top referenced anti-Insulin antibody for Western Blotting

Similar anti-Insulin Antibodies

Application / Reactivity Pig (Porcine)
Dot Blot (DB) 2 Antibodies
ELISA 149 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 14 Antibodies
Flow Cytometry (FACS) 117 Antibodies
Functional Studies (Func) 12 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 73 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 50 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 122 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 199 Antibodies
Immunoprecipitation (IP) 55 Antibodies

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Pig (Porcine)
(897), (376), (253), (246), (240), (119), (49), (5), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(812), (178), (57), (7), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(55), (41), (40), (27), (25), (24), (19), (19), (17), (17), (17), (17), (17), (17), (17), (17), (16), (16), (12), (10), (7), (7), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(582), (345), (231), (203), (187), (178), (149), (128), (84), (56), (52), (27), (12), (12), (9), (8), (7), (6), (5), (5), (4), (3), (2), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Specificity The antibody recognizes Insulin and recombinant Proinsulin, but does not react with synthetic C-Peptide.
Characteristics Synonyms: INS
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant full length Insulin expressed in Yeast.
Clone HPI-B5
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood sugar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations.Synonyms: INS
Gene ID 3630
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Indirect ELISA: Use at 2 μg/mL (For detection of antigen). Western Blot: Use at 4-5 μg/mL. Immunohistochemistry: Use at 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °CAvoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Insulin antibody (INS) (ABIN487416) anti-Insulin (INS) antibody
Western Blotting (WB) image for anti-Insulin antibody (INS) (ABIN487416) anti-Insulin (INS) antibody (Image 2)