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anti-Mouse (Murine) Insulin antibody for Immunocytochemistry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Epitope
AA 25-110
27
20
15
14
12
10
6
6
5
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
916
394
268
264
263
121
49
6
6
5
5
5
4
3
2
1
1
1
1
1
1
1
Host
Mouse
812
175
54
9
2
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
55
42
41
27
24
24
19
19
18
17
17
17
17
17
17
16
16
12
11
10
7
7
7
5
5
5
5
5
5
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC)
614
360
243
219
205
185
157
135
69
66
38
28
14
11
9
9
8
7
5
4
3
2
2
2
2
1
1
Supplier
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Catalog No. ABIN4899865
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.274792 ABIN4900790 FACS ICC Rat IgG2a Log in to see 182410 5
26.274792 ABIN4899864 FACS ICC Biotin Mouse IgG2a AA 25-110 Log in to see 253627
1 ABIN617877 IEM ICC IF IHC Rabbit IgG Log in to see Polyclonal 44
1 ABIN337249 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN1859459 ICC IHC IP WB Rabbit IgG AA 25-54, AA 90-110 Log in to see Polyclonal
1 ABIN2470654 ICC FACS IF IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1735946 ICC FACS IF IHC (p) ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326068 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326072 ELISA ICC IF IHC (fro) IHC (p) WB Biotin Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326070 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326073 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326087 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326092 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326099 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326077 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326067 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326054 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326075 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)

Top referenced anti-Insulin antibody for Immunocytochemistry

Similar anti-Insulin Antibodies

Application / Reactivity Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 87 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 124 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 61 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 75 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 92 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 152 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Protein Assay (PrA) 2 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 86 Antibodies

General

Antigen Insulin (INS) Antibodies
Epitope AA 25-110
(27), (20), (15), (14), (12), (10), (6), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(916), (394), (268), (264), (263), (121), (49), (6), (6), (5), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(812), (175), (54), (9), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(55), (42), (41), (27), (24), (24), (19), (19), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (10), (7), (7), (7), (5), (5), (5), (5), (5), (5), (2), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC)
(614), (360), (243), (219), (205), (185), (157), (135), (69), (66), (38), (28), (14), (11), (9), (9), (8), (7), (5), (4), (3), (2), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN4899865) Images
Specificity Detects human and mouse Proinsulin. Does not detect mature insulin.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Proinsulin, Phe25-Asn110, Accession # P01308
Clone 253627
Isotype IgG2a

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin Antibody (ABIN4899865) Images back to top
Antigen
Alternative Name Proinsulin (INS Antibody Abstract)
Background Alternative Names: IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin
Gene ID 3630
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin Antibody (ABIN4899865) Images back to top
Application Notes Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin Antibody (ABIN4899865) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Insulin Antibody (ABIN4899865)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Jonatan, Spence, Method, Kofron, Sinagoga, Haataja, Arvan, Deutsch, Wells: "Sox17 regulates insulin secretion in the normal and pathologic mouse β cell." in: PLoS ONE, Vol. 9, Issue 8, pp. e104675, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Cao, Mao, Kam, Xiao, Cao, Shen, Cheng, Xu, Lee, Jiang, Xia: "PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance." in: PLoS biology, Vol. 11, Issue 4, pp. e1001541, 2013

Chen, Begum, Opare-Addo, Garyu, Gibson, Bothwell, Papaioannou, Herold: "Promotion of beta-cell differentiation in pancreatic precursor cells by adult islet cells." in: Endocrinology, Vol. 150, Issue 2, pp. 570-9, 2009 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN4899865) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Insulin antibody (INS) (AA 25-110) (ABIN4899865) Proinsulin in beta TC-6 Mouse Cell Line. Proinsulin was detected in immersion fixed b...