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anti-Mouse (Murine) Insulin antibody for Immunocytochemistry

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Epitope
AA 25-110
28
20
13
12
12
10
8
8
6
4
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
902
373
250
242
241
116
49
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
819
176
57
9
2
2
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
54
40
40
26
24
24
19
19
18
17
17
17
17
17
17
16
16
11
11
9
7
7
5
5
5
5
5
2
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC)
623
357
228
212
210
192
158
144
60
56
43
28
14
12
11
9
8
7
6
5
5
4
3
2
1
1
Supplier
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Catalog No. ABIN4899865
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.37591 ABIN4900790 FACS ICC Rat IgG2a Log in to see 182410 5
26.37591 ABIN617877 IEM ICC IF IHC Rabbit IgG Log in to see Polyclonal 44
26.37591 ABIN4899864 FACS ICC Biotin Mouse IgG2a AA 25-110 Log in to see 253627
1 ABIN1859459 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 25-54, AA 90-110 Log in to see Polyclonal
1 ABIN2470654 ICC FACS IF IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN4326054 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326053 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326057 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326089 ICC IF IHC (fro) IHC (p) WB Biotin Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326090 FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326091 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326092 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326094 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326097 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326098 FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326099 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326067 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)

Top referenced anti-Insulin antibody for Immunocytochemistry

Similar anti-Insulin Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 10 Antibodies 14 Antibodies
ELISA 81 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 123 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 60 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 74 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 89 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 147 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Protein Assay (PrA) 2 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Western Blotting (WB) 77 Antibodies

General

Antigen Insulin (INS) Antibodies
Epitope AA 25-110
(28), (20), (13), (12), (12), (10), (8), (8), (6), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(902), (373), (250), (242), (241), (116), (49), (5), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(819), (176), (57), (9), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(54), (40), (40), (26), (24), (24), (19), (19), (18), (17), (17), (17), (17), (17), (17), (16), (16), (11), (11), (9), (7), (7), (5), (5), (5), (5), (5), (2), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC)
(623), (357), (228), (212), (210), (192), (158), (144), (60), (56), (43), (28), (14), (12), (11), (9), (8), (7), (6), (5), (5), (4), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN4899865) Images
Specificity Detects human and mouse Proinsulin. Does not detect mature insulin.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Proinsulin, Phe25-Asn110, Accession # P01308
Clone 253627
Isotype IgG2a

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling References for anti-Insulin Antibody (ABIN4899865) Images back to top
Antigen
Alternative Name Proinsulin (INS Antibody Abstract)
Gene ID 3630
UniProt P01308
Research Area Hormones, Neurology
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin Antibody (ABIN4899865) Images back to top
Application Notes Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin Antibody (ABIN4899865) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Insulin Antibody (ABIN4899865)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Jonatan, Spence, Method, Kofron, Sinagoga, Haataja, Arvan, Deutsch, Wells: "Sox17 regulates insulin secretion in the normal and pathologic mouse β cell." in: PLoS ONE, Vol. 9, Issue 8, pp. e104675, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Cao, Mao, Kam, Xiao, Cao, Shen, Cheng, Xu, Lee, Jiang, Xia: "PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance." in: PLoS biology, Vol. 11, Issue 4, pp. e1001541, 2013

Chen, Begum, Opare-Addo, Garyu, Gibson, Bothwell, Papaioannou, Herold: "Promotion of beta-cell differentiation in pancreatic precursor cells by adult islet cells." in: Endocrinology, Vol. 150, Issue 2, pp. 570-9, 2009 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN4899865) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Insulin antibody (INS) (AA 25-110) (ABIN4899865) Proinsulin in beta TC-6 Mouse Cell Line. Proinsulin was detected in immersion fixed b...