anti-Mouse (Murine) Insulin antibody for Immunofluorescence

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Human, Mouse (Murine), Rat (Rattus)
949
397
283
274
261
121
49
6
6
5
5
5
4
2
2
1
1
1
1
1
1
1
Host
Rabbit
836
181
54
16
3
1
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
56
42
41
27
26
26
21
21
18
17
17
17
17
17
17
16
16
12
11
10
9
9
9
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
622
371
247
233
205
203
160
138
70
66
40
27
16
11
9
8
8
7
5
4
3
3
3
2
2
2
1
1
1
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Catalog No. ABIN372838
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN617877 IEM ICC IF IHC Rabbit IgG Log in to see Polyclonal 44
1 ABIN337249 FACS ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN4326144 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4326145 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4326146 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4326053 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326054 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326057 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326141 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794
1 ABIN4326142 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794
1 ABIN4326143 FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-794
1 ABIN4326149 CyTOF FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4326150 CyTOF FACS IF IHC (p) Mouse IgG1 kappa Log in to see IRDN-805
1 ABIN4904068 ICC IF IHC Rabbit IgG Log in to see
1 ABIN1735946 ICC FACS IF IHC (p) ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN4326067 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326068 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(949), (397), (283), (274), (261), (121), (49), (6), (6), (5), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(836), (181), (54), (16), (3), (1)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(56), (42), (41), (27), (26), (26), (21), (21), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(622), (371), (247), (233), (205), (203), (160), (138), (70), (66), (40), (27), (16), (11), (9), (8), (8), (7), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) Images
Specificity This antibody recognizes endogenous levels of total Human Insulin protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Immunogen INS antibody was raised against Synthetic peptide (KLH-coupled) derived from the sequence of Human Insulin.
Isotype IgG

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) Images back to top
Application Notes ELISA. Flow Cytometry. Immunofluorescence. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details ProductDetails: References for anti-Insulin antibody (ABIN372838) Images back to top
Format Liquid
Concentration 0.02 mg/mL
Buffer 10 mM HEPES, pH 7.5, 150 mM Sodium Chloride, 100 μg/mL BSA, 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-Insulin antibody (ABIN372838)

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Product cited in:

Micucci, Orciari, Catalano: "Hyperglycemia promotes K-Ras-induced lung tumorigenesis through BASCs amplification." in: PLoS ONE, Vol. 9, Issue 8, pp. e105550, 2014

Madsen, Knauf, Gotfredsen, Pilling, Sjögren, Andersen, Andersen, de Boer, Manova, Barlas, Vundavalli, Nyborg, Knudsen, Moelck, Fagin: "GLP-1 receptor agonists and the thyroid: C-cell effects in mice are mediated via the GLP-1 receptor and not associated with RET activation." in: Endocrinology, Vol. 153, Issue 3, pp. 1538-47, 2012

"Retraction." in: Endocrinology, 2010

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling ProductDetails: References for anti-Insulin antibody (ABIN372838) back to top
Supplier Images
 image for anti-Insulin (INS) antibody (ABIN372838) anti-Insulin (INS) antibody