|Application / Reactivity||Pig (Porcine)|
|Cytometry by Time of Flight (CyTOF)||12 Antibodies|
|Dot Blot (DB)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||14 Antibodies|
|Flow Cytometry (FACS)||137 Antibodies|
|Functional Studies (Func)||9 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||89 Antibodies|
|Immunoelectron Microscopy (IEM)||1 Antibodies|
|Immunofluorescence (IF)||108 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||88 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||148 Antibodies|
|Antigen||Insulin (INS) Antibodies|
|Reactivity||Pig (Porcine) Alternatives|
|Host||Guinea Pig Alternatives|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Immunohistochemistry (Acetone-fixed) (IHC (af)), ELISA
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Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling Images
Guinea pig anti Pig insulin antibody recognizes porcine insulin, a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin belongs to a family of hormones including the insulin-like growth hormones and the relaxins. The insulin precursor is a 108amino acid polypeptide consisting of a 24 aa signal peptide, an insulin A chain, a prohormone polypeptide (also known as the 'C chain') and an insulin B chain together having a predicted molecular mass of 11,672 Da. Active insulin migrates with an apparent molecular mass of 6 kDa in western blotting of porcine, bovine and lapine samples (Schechter et al. 1992).
Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver, it induces uptake of glucose into cells through its interaction with the glucose transporter GLUT4 (Watson and Pressin 2001). Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma (Schiel et al. 2006).
Guinea pig anti pig insulin antibody has been successfully used to demonstrate the presence og insulin in the islets of Langerhans of murine pancreas using immunohistochemistry on paraffin embedded material (Ortsater et al. 2012).
GUINEA PIG ANTI PIG INSULIN
Protein size: 108
|Immunogen||The immunogen for anti-INS antibody: native|
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling Images back to top
|Alternative Name||INS (INS Antibody Abstract)|
|Pathways||NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.