anti-Pig (Porcine) Insulin antibody for Immunoprecipitation

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Human, Pig (Porcine)
965
396
302
283
262
121
49
6
6
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
842
183
54
25
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
57
42
41
28
28
27
23
23
18
17
17
17
17
17
17
16
16
12
11
11
11
11
11
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
622
372
248
233
208
204
161
142
70
66
40
27
18
11
9
8
8
7
5
4
3
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN5556681
$ 411.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139
1 ABIN1859457 ICC IHC IP WB Rabbit IgG AA 88-108 Log in to see Polyclonal
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326053 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326054 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326057 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326059 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326060 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326062 CyTOF FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326061 CyTOF FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN2742155 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 1-84 Log in to see 2D11-H5 or INS05
1 ABIN4326101 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326107 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326028 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326103 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326109 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326106 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SPM139
1 ABIN4326112 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM139

General

Antigen Insulin (INS) Antibodies
Reactivity Human, Pig (Porcine)
(965), (396), (302), (283), (262), (121), (49), (6), (6), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(842), (183), (54), (25), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(57), (42), (41), (28), (28), (27), (23), (23), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (11), (11), (11), (11), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(622), (372), (248), (233), (208), (204), (161), (142), (70), (66), (40), (27), (18), (11), (9), (8), (8), (7), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Sequence Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
Specificity This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
Characteristics Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
Purification Protein A Chromatography
Immunogen Purified human Insulin coupled to Bovine Serum Albumin (BSA).
Clone 2D11-H5
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
Molecular Weight 6 kDa
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Diabetes, Hormones, Neurology
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10 μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imately 5 ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.