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anti-Pig (Porcine) Insulin antibody for Western Blotting

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Reactivity
Cow (Bovine), Human, Pig (Porcine)
562
134
134
97
77
25
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Host
Mouse
446
177
57
2
2
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
37
28
25
12
10
10
6
5
5
5
5
5
5
5
5
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
385
181
140
123
67
56
49
42
39
36
25
13
12
12
9
8
7
6
5
5
4
3
1
1
1
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Catalog No. ABIN487416
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.123033054 ABIN411565 IHC ELISA WB Mouse IgG1 full length Log in to see HPI-B5
0.123033054 ABIN1859457 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 88-108 Log in to see Polyclonal
0.123033054 ABIN1859458 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 25-54 Log in to see Polyclonal
0.0011806352 ABIN117970 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 2D11-H5 1
0.0011806352 ABIN272321 EIA IHC (p) WB Mouse IgG1 Log in to see E2E3 3
0.0011806352 ABIN1832566 ELISA WB FITC Guinea Pig IgG Log in to see Polyclonal
0.0011806352 ABIN1832565 ELISA WB Guinea Pig IgG Log in to see Polyclonal
0.0011806352 ABIN2610269 ELISA WB Biotin Rabbit IgG AA 25-54 Log in to see Polyclonal
0.0011806352 ABIN2610271 ELISA WB Biotin Rabbit IgG AA 88-108 Log in to see Polyclonal
0.0011806352 ABIN2610272 WB FITC Rabbit IgG AA 25-54 Log in to see Polyclonal
0.0011806352 ABIN2610274 WB FITC Rabbit IgG AA 88-108 Log in to see Polyclonal
0.0011806352 ABIN1585829 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0011806352 ABIN201810 IHC ELISA WB Mouse IgG1 Log in to see
0.0011806352 ABIN2294164 IC IHC ELISA WB Rabbit IgG AA 88-108 Log in to see Polyclonal
0.0011806352 ABIN2294244 ELISA WB FITC Guinea Pig IgG Log in to see Polyclonal
0.0011806352 ABIN458570 ICC ELISA WB Guinea Pig IgG Log in to see Polyclonal
0.0011806352 ABIN307024 WB Mouse IgG1 Log in to see E2E3 3
0.0011806352 ABIN2294242 ELISA WB Guinea Pig IgG Log in to see Polyclonal

Top referenced anti-Insulin antibody for Western Blotting

Similar anti-Insulin Antibodies

Application / Reactivity Pig (Porcine)
Dot Blot (DB) 2 Antibodies
ELISA 68 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Functional Studies (Func) 13 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Pig (Porcine)
(562), (134), (134), (97), (77), (25), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(446), (177), (57), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(37), (28), (25), (12), (10), (10), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(385), (181), (140), (123), (67), (56), (49), (42), (39), (36), (25), (13), (12), (12), (9), (8), (7), (6), (5), (5), (4), (3), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Specificity The antibody recognizes Insulin and recombinant Proinsulin, but does not react with synthetic C-Peptide.
Characteristics Synonyms: INS
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant full length Insulin expressed in Yeast.
Clone HPI-B5
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood sugar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations.Synonyms: INS
Gene ID 3630
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Indirect ELISA: Use at 2 μg/mL (For detection of antigen). Western Blot: Use at 4-5 μg/mL. Immunohistochemistry: Use at 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °CAvoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Insulin antibody (INS) (ABIN487416) anti-Insulin (INS) antibody
Western Blotting (WB) image for anti-Insulin antibody (INS) (ABIN487416) anti-Insulin (INS) antibody (Image 2)