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anti-Wild boar (Sus scrofa) Insulin antibody for Western Blotting

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Epitope
AA 88-108
28
20
14
13
12
11
11
10
9
5
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Wild boar (Sus scrofa), Pig (Porcine)
887
368
249
238
235
118
48
4
4
3
3
3
3
2
1
1
1
1
1
1
1
1
Host
Rabbit
797
179
57
7
2
2
Clonality
Polyclonal
Conjugate
This Insulin antibody is un-conjugated
55
41
40
27
25
24
19
19
17
17
17
17
17
17
17
17
16
16
12
10
7
7
5
5
5
5
5
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
569
340
233
196
182
180
150
126
85
56
52
27
13
12
9
8
7
6
5
5
4
3
1
1
1
Supplier
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Catalog No. ABIN1859457
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN1859458 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 25-54 Log in to see Polyclonal

Similar anti-Insulin Antibodies

Application / Reactivity Wild boar (Sus scrofa) Pig (Porcine)
ELISA 2 Antibodies 148 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 73 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Western Blotting (WB) 2 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 117 Antibodies
Functional Studies (Func) 13 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies

General

Antigen Insulin (INS) Antibodies
Epitope AA 88-108
(28), (20), (14), (13), (12), (11), (11), (10), (9), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Wild boar (Sus scrofa), Pig (Porcine)
(887), (368), (249), (238), (235), (118), (48), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(797), (179), (57), (7), (2), (2)
Clonality
Polyclonal
Conjugate This Insulin antibody is un-conjugated
(55), (41), (40), (27), (25), (24), (19), (19), (17), (17), (17), (17), (17), (17), (17), (17), (16), (16), (12), (10), (7), (7), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(569), (340), (233), (196), (182), (180), (150), (126), (85), (56), (52), (27), (13), (12), (9), (8), (7), (6), (5), (5), (4), (3), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Sequence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -GIV EQCCTSICSL YQLENYCN
Specificity It has been selected for its ability to recognize INS in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen INS (AA 88-108)
Isotype IgG

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant INS (Gly88~Asn108) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Insulin antibody (INS) (AA 88-108) (ABIN1859457) anti-Insulin (INS) (AA 88-108) antibody