Goat Insulin ELISA Kit

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Antigen
Insulin (INS) ELISA Kits
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
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  • Ins
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  • LOC100060077
Reactivity
Goat
Alternatives
Kits with alternative reactivity to:
62
32
30
22
15
15
13
13
10
9
8
6
6
3
2
1
1
Method Type
Competition ELISA
Detection Range
123.5-10000 pg/mL
Minimum Detection Limit
123.5 pg/mL
Application
ELISA
Options
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Catalog No. ABIN5564663
$ 651.79
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN857454 Sandwich ELISA Serum, Plasma, Tissue Homogenate 10-100 μIU/mL 10 μIU/mL Log in to see
1 ABIN993082 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN5520028 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN4986989 Sandwich ELISA Plasma, Tissue Homogenate, Serum 8-140 μIU/mL 8 μIU/mL Log in to see
1 ABIN4970141 Sandwich ELISA 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5074460 Log in to see

General

Antigen Insulin (INS) ELISA Kits
Reactivity Goat
Kits with alternative reactivity to:
(62), (32), (30), (22), (15), (15), (13), (13), (10), (9), (8), (6), (6), (3), (2), (1), (1)
Method Type Competition ELISA
Detection Range 123.5-10000 pg/mL
Minimum Detection Limit 123.5 pg/mL
Application ELISA
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Product Details Insulin ELISA Kit

Target details Application Details Handling Images
Sample Type Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity

This assay has high sensitivity and excellent specificity for detection of Insulin (INS).
No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.

Sensitivity 52.4 pg/mL
Material not included
  • Microplate reader with 450 nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution

Target details

Product Details Insulin ELISA Kit Application Details Handling Images back to top
Antigen
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details Insulin ELISA Kit Target details Handling Images back to top
Assay Time 2 h
Plate Pre-coated
Protocol This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample.
Assay Procedure
  1. Prepare all reagents, samples and standards,
  2. Add 50μL standard or sample to each well.
    And then add 50μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37 °C,
  3. Aspirate and wash 3 times,
  4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
  5. Aspirate and wash 5 times,
  6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
  7. Add 50μL Stop Solution. Read at 450 nm immediately.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin (INS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin (INS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only

Handling

Product Details Insulin ELISA Kit Target details Application Details Images back to top
Handling Advice

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage 4 °C

Images

Product Details Insulin ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Insulin (INS) ELISA Kit (ABIN5564663) Insulin (INS) ELISA Kit