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Human Insulin ELISA Kit

Recommended Insulin Product (supplied by: Log in to see )

Antigen
Insulin (INS) ELISA Kits
  • AA986540
  • IDDM2
  • ILPR
  • Ins-2
  • ins1
  • ins1-a
  • InsII
  • Insulin
  • IRDN
  • Mody
  • Mody4
  • MODY10
  • proinsulin
  • xins
  • zgc:109842
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
50
27
25
19
16
16
13
13
11
7
7
6
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
1.87-120 μIU/mL
Minimum Detection Limit
1.87 μIU/mL
Application
ELISA
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Catalog No. ABIN2506949
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32670403 ABIN1115510 Competition ELISA Serum, Plasma, Biological Fluids 39.063-2500 pg/mL 39.06 pg/mL Log in to see
0.32670403 ABIN1116658 Sandwich ELISA Serum, Plasma, Biological Fluids 1.563-100 fM/mL 0.31 ng/mL Log in to see
0.32670403 ABIN455435 Serum, Plasma, Biological Fluids 1.56-100 MioU/L 1.56 MioU/L Log in to see
0.32670403 ABIN1029634 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 12.5-800 pg/mL 12.5 pg/mL Log in to see
0.32670403 ABIN2762391 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 156.25-10000 pg/mL 156.25 pg/mL Log in to see
0.001210097 ABIN816535 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 8-140 μIU/mL 8 μIU/mL Log in to see 1
0.001210097 ABIN1873331 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Biological Fluids 61.73-5000 pg/mL 61.73 pg/mL Log in to see 4
0.001210097 ABIN3032953 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.7-300 μIU/mL 4.7 μIU/mL Log in to see
0.001210097 ABIN649043 Sandwich ELISA Log in to see 9
0.001210097 ABIN625032 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 8
0.001210097 ABIN2014348 Sandwich ELISA Serum, Plasma 0.1817-138 mU/L 0.1817 mU/L Log in to see
0.001210097 ABIN511390 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 10-250 mIU/mL 10 mIU/mL Log in to see
0.001210097 ABIN1057574 Sandwich ELISA Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma 5-100 pM/L 5 pM/L Log in to see
0.001210097 ABIN456245 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 3.12-200 pM/L 3.12 pM/L Log in to see
0.001210097 ABIN1813709 Sandwich ELISA Body Fluids, Tissue Lysate, Cell Culture Supernatant, Serum Log in to see 3
0.001210097 ABIN2684752 Sandwich ELISA Plasma, Serum 6.25-100 μIU/mL 6.25 μIU/mL Log in to see 1
0.001210097 ABIN511474 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
0.001210097 ABIN365877 Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.001210097 ABIN1028893 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 39.063-2500 pg/mL 39.063 pg/mL Log in to see
0.001210097 ABIN510994 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see

Top referenced Insulin ELISA Kit for ELISA

  • Human Insulin ELISA Kit for Sandwich ELISA - ABIN625032 (8 Publications): Leisegang, Bouic, Menkveld, Henkel: Obesity is associated with increased seminal insulin and leptin alongside reduced fertility parameters in a controlled male cohort. in Reproductive biology and endocrinology : RB&E 2014 (PubMed)

  • Human Insulin ELISA Kit for Sandwich ELISA - ABIN649043 (9 Publications): Gerbitz et al.: [Pancreatic B cell peptides: kinetic behaviour and concentrations of proinsulin, insulin and C-peptide in plasma and urine, problems of assay methods, clinical significance and literature review ... in Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie 1981 (PubMed)

  • Human Insulin ELISA Kit for Competition ELISA - ABIN1873331 (4 Publications): Tiunov, Kustov, Arutiunian, Belyĭ, Gudiev: [Data regarding the toxicology of CO]. in Gigiena truda i professional'nye zabolevaniia 1986 (PubMed)

  • Human Insulin ELISA Kit - ABIN1326860 (2 Publications): Bakry, Shoeib, El Shafiee, Hassan: Androgenetic alopecia, metabolic syndrome, and insulin resistance: Is there any association? A case-control study. in Indian dermatology online journal 2014 (PubMed)

  • Human Insulin ELISA Kit for Sandwich ELISA - ABIN816535 (1 Publications): Ortega, Fernández-Elías, Hamouti, Mora-Rodriguez: Increased blood cholesterol after a high saturated fat diet is prevented by aerobic exercise training. in Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme 2013 (PubMed)

  • Human Insulin ELISA Kit for Sandwich ELISA - ABIN2684752 (1 Publications): Wang, Zhan, Li, Yu, Liu: Identification and validation co-differentially expressed genes with NAFLD and insulin resistance. in Endocrine 2015 (PubMed)

Similar Insulin ELISA Kits

Application / Reactivity Human
ELISA 47 ELISA Kits

General

Antigen Insulin (INS) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(50), (27), (25), (19), (16), (16), (13), (13), (11), (7), (7), (6), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 1.87-120 μIU/mL
Minimum Detection Limit 1.87 μIU/mL
Application ELISA
Supplier Log in to see

Product Details Insulin ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Insulin in human serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of human Insulin
Sensitivity <1 μIU/mL
Characteristics one wash
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • human Insulin Mix-4x 9 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details Insulin ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS ELISA Kit Abstract)
Background Insulin is a peptide hormone produced by beta cells in the pancreas. It regulates the metabolism of carbohydrates and fats by promoting the absorption of glucose from the blood to skeletal muscles and fat tissue and by causing fat to be stored rather than used for energy.
Molecular Weight 5808 Da
Gene ID 3630
NCBI Accession NP_000198
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling

Application Details

Product Details Insulin ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 1.5 h
Plate Strips (12 x 8)
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for insulin has been pre-coated onto a microplate. Standards, samples and human insulin mix are pipetted into the wells and any insulin present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of insulin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 490 μL 1x Assay Diluent to prepare a 300 μIU/mL standard. Gently vortex to mix.Take 200 μL Insulin standard into a tube, then add 300 μL 1x Assay Diluent to prepare a 120 μIU/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 150 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. human Insulin Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1490 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:2.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of Insulin Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 1 hour at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

Product Details Insulin ELISA Kit Target details Application Details Images back to top
Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

Product Details Insulin ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Insulin ELISA Kit (INS) (ABIN2506949) Insulin (INS) ELISA Kit