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|Antigen||Insulin Receptor (INSR) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Insulin Receptor antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Insulin Receptor AntibodyTarget Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor antibody (ABIN968448) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details Insulin ReceptorProduct Details anti-Insulin Receptor Antibody Application Details Handling References for anti-Insulin Receptor antibody (ABIN968448) Images back to top
|Alternative Name||CD220 (INSR Antibody Abstract)|
The Insulin Receptor (IR), also known as CD220, is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa alpha-subunit with kinase activity and a 95 kDa beta-chain. The active Insulin Receptor is a heterotetramer of homologous alpha and beta subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, beta-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling.
This antibody was generated using the human insulin pro-receptor beta-subunit aa. 1212-1381.
|Molecular Weight||95 kDa|
|Pathways||NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity|
Application DetailsProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling References for anti-Insulin Receptor antibody (ABIN968448) Images back to top
Related Products: ABIN968548, ABIN967390
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details References for anti-Insulin Receptor antibody (ABIN968448) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
References for anti-Insulin Receptor antibody (ABIN968448)Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling Images back to top
|Product cited in:||
Parpal, Karlsson, Thorn, Strålfors: "Cholesterol depletion disrupts caveolae and insulin receptor signaling for metabolic control via insulin receptor substrate-1, but not for mitogen-activated protein kinase control." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 9670-8, 2001
Swindle, Tran, Johnson, Banerjee, Mayes, Griffith, Wells: "Epidermal growth factor (EGF)-like repeats of human tenascin-C as ligands for EGF receptor." in: The Journal of cell biology, Vol. 154, Issue 2, pp. 459-68, 2001
Kasus-Jacobi, Béréziat, Perdereau, Girard, Burnol: "Evidence for an interaction between the insulin receptor and Grb7. A role for two of its binding domains, PIR and SH2." in: Oncogene, Vol. 19, Issue 16, pp. 2052-9, 2000
Lu, Guidotti: "Identification of the cysteine residues involved in the class I disulfide bonds of the human insulin receptor: properties of insulin receptor monomers." in: Molecular biology of the cell, Vol. 7, Issue 5, pp. 679-91, 1997
White, Kahn: "The insulin signaling system." in: The Journal of biological chemistry, Vol. 269, Issue 1, pp. 1-4, 1994
ImagesProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor antibody (ABIN968448) back to top