You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Insulin Receptor antibody for Immunofluorescence

Recommended Insulin Receptor Antibody (supplied by: Log in to see )

Antigen
Insulin Receptor (INSR) Antibodies
  • 18402
  • 4932439J01Rik
  • CD220
  • cd220
  • CG18402
  • CTK-1
  • D630014A15Rik
  • DIHR
  • DILR
  • dInR
  • dINR
  • dinr
  • dInr
  • DInR
  • DInr
  • dInsR
  • dir
  • DIR
  • dIR
  • Dir-a
  • Dir-b
  • DIRbeta
  • dIRH
  • DIRH
  • Dmel\\CG18402
  • er10
  • HHF5
  • hhf5
  • Inr
  • INR
  • inr
  • Inr-alpha
  • Inr-beta
  • INS
  • INSR
  • InsR
  • insulin/insulin-like growth factor receptor
  • ir
  • IR
  • IR-A
  • ir-A
  • IR-B
  • l(3)05545
  • l(3)93Dj
  • l(3)er10
  • lnR
  • NV14476
Reactivity
Human, Mouse (Murine), Rat (Rattus)
265
80
56
11
8
5
4
2
1
1
1
1
1
1
1
Host
Rabbit
218
76
3
Clonality
Polyclonal
Conjugate
This Insulin Receptor antibody is un-conjugated
21
16
9
9
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
167
122
50
43
35
19
18
11
11
10
10
8
7
7
2
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN968448
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3161046 ABIN2490412 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.3161046 ABIN2490881 IF ELISA Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN2888665 IF ELISA Rabbit IgG AA 1326-1375 Log in to see Polyclonal
0.0010850558 ABIN2755564 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.0010850558 ABIN3198164 IC IF IHC WB Rabbit C-Term, pTyr1361 Log in to see Polyclonal
0.0010850558 ABIN1173903 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN2972579 ICC IF WB Rabbit C-Term, pTyr1361 Log in to see Polyclonal
0.0010850558 ABIN3066099 ICC IF IHC WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-Insulin Receptor antibody for Immunofluorescence

Similar anti-Insulin Receptor Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 146 Antibodies 66 Antibodies 44 Antibodies
Neutralization (Neut) 11 Antibodies 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 52 Antibodies 9 Antibodies 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies 6 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 36 Antibodies 7 Antibodies 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Insulin Receptor (INSR) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(265), (80), (56), (11), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(218), (76), (3)
Clonality
Polyclonal
Conjugate This Insulin Receptor antibody is un-conjugated
(21), (16), (9), (9), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(167), (122), (50), (43), (35), (19), (18), (11), (11), (10), (10), (8), (7), (7), (2), (2), (2), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-Insulin Receptor Antibody

Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor Antibody (ABIN968448) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Isotype Ig

Target Details Insulin Receptor

Product Details anti-Insulin Receptor Antibody Application Details Handling References for anti-Insulin Receptor Antibody (ABIN968448) Images back to top
Antigen
Alternative Name CD220 (INSR Antibody Abstract)
Background The Insulin Receptor (IR), also known as CD220, is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa alpha-subunit with kinase activity and a 95 kDa beta-chain. The active Insulin Receptor is a heterotetramer of homologous alpha and beta subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, beta-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling.
This antibody was generated using the human insulin pro-receptor beta-subunit aa. 1212-1381.
Molecular Weight 95 kDa
Pathways NF-kappaB Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling References for anti-Insulin Receptor Antibody (ABIN968448) Images back to top
Comment

Related Products: ABIN968548, ABIN967390

Restrictions For Research Use only

Handling

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details References for anti-Insulin Receptor Antibody (ABIN968448) Images back to top
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-Insulin Receptor Antibody (ABIN968448)

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling Images back to top
Product cited in:

Parpal, Karlsson, Thorn, Strålfors: "Cholesterol depletion disrupts caveolae and insulin receptor signaling for metabolic control via insulin receptor substrate-1, but not for mitogen-activated protein kinase control." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 9670-8, 2001

Swindle, Tran, Johnson, Banerjee, Mayes, Griffith, Wells: "Epidermal growth factor (EGF)-like repeats of human tenascin-C as ligands for EGF receptor." in: The Journal of cell biology, Vol. 154, Issue 2, pp. 459-68, 2001

Kasus-Jacobi, Béréziat, Perdereau, Girard, Burnol: "Evidence for an interaction between the insulin receptor and Grb7. A role for two of its binding domains, PIR and SH2." in: Oncogene, Vol. 19, Issue 16, pp. 2052-9, 2000

Lu, Guidotti: "Identification of the cysteine residues involved in the class I disulfide bonds of the human insulin receptor: properties of insulin receptor monomers." in: Molecular biology of the cell, Vol. 7, Issue 5, pp. 679-91, 1997

White, Kahn: "The insulin signaling system." in: The Journal of biological chemistry, Vol. 269, Issue 1, pp. 1-4, 1994

Images

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor Antibody (ABIN968448) back to top
Supplier Images
Western Blotting (WB) image for anti-Insulin Receptor antibody (INSR) (ABIN968448) Western blot analysis of CD220 (Insulin receptor beta) on a rat liver lysate. Lane 1:...
Immunofluorescence (IF) image for anti-Insulin Receptor antibody (INSR) (ABIN968448) Immunofluorescence staining of rat neurons.