anti-Rat (Rattus) Insulin Receptor antibody for Enzyme Immunoassay

Recommended Insulin Receptor Antibody (supplied by: Log in to see )

Antigen
Insulin Receptor (INSR) Antibodies
  • 18402
  • CG18402
  • DIHR
  • DILR
  • DIR
  • DIRH
  • DIRbeta
  • DInR
  • DInr
  • Dir-a
  • Dir-b
  • Dmel\\CG18402
  • INR
  • INS
  • IR
  • Inr
  • Inr-alpha
  • Inr-beta
  • InsR
  • dINR
  • dIR
  • dIRH
  • dInR
  • dInr
  • dInsR
  • dinr
  • dir
  • er10
  • inr
  • insulin/insulin-like growth factor receptor
  • l(3)05545
  • l(3)93Dj
  • l(3)er10
  • lnR
  • INSR
  • NV14476
  • CD220
  • HHF5
  • 4932439J01Rik
  • D630014A15Rik
  • IR-A
  • IR-B
  • ir-A
  • ir
  • cd220
  • hhf5
  • CTK-1
  • Insulin-like receptor
  • insulin receptor
  • insulin receptor precursor
  • InR
  • INSR
  • Insr
  • insr
  • DAF-2
Epitope
Beta Chain
38
34
22
17
17
14
12
10
9
8
7
6
6
5
5
5
5
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
312
127
93
23
21
21
20
17
17
9
5
5
3
1
1
1
1
1
Host
Mouse
252
85
8
1
1
Clonality
Monoclonal
Conjugate
This Insulin Receptor antibody is un-conjugated
26
18
12
11
9
9
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
214
143
73
57
46
32
17
13
13
12
9
8
7
6
4
3
2
2
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Catalog No. ABIN126821
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126820 EIA IHC (fro) WB Mouse IgG1 Beta Chain Log in to see 9H4

General

Antigen Insulin Receptor (INSR) Antibodies
Epitope Beta Chain
(38), (34), (22), (17), (17), (14), (12), (10), (9), (8), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(312), (127), (93), (23), (21), (21), (20), (17), (17), (9), (5), (5), (3), (1), (1), (1), (1), (1)
Host Mouse
(252), (85), (8), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin Receptor antibody is un-conjugated
(26), (18), (12), (11), (9), (9), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(214), (143), (73), (57), (46), (32), (17), (13), (13), (12), (9), (8), (7), (6), (4), (3), (2), (2)
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Product Details anti-Insulin Receptor Antibody

Target Details Insulin Receptor Application Details Handling Images
Specificity This antibody specifically recognizes the C-terminus of Insulin receptor (phosphorylation-independent).
Characteristics Synonyms: Insulin Receptor
Purification Subsequent thiophilic adsorption and size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic peptide conjugated to KLH
Clone 11B6
Isotype IgG1

Target Details Insulin Receptor

Product Details anti-Insulin Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD220 / INSR (INSR Antibody Abstract)
Background The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.Synonyms: Insulin Receptor
Gene ID 3643
UniProt P06213
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity

Application Details

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Cell LysateFormulation: Lyophilized cell lysate from serum starved HepG2 cells. Recostitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Insulin Receptor (INSR) (Beta Chain) antibody (ABIN126821) anti-Insulin Receptor (INSR) (Beta Chain) antibody