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anti-Dog (Canine) Integrin beta 2 antibody for Flow Cytometry

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Reactivity
Dog (Canine), Human
466
209
111
39
38
21
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rat
363
186
155
5
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is conjugated to Biotin
92
77
52
19
14
11
9
8
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Flow Cytometry (FACS)
497
275
273
158
105
105
84
62
44
34
20
9
7
6
5
3
2
1
1
1
1
1
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Catalog No. ABIN118551
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN118555 FACS PE Rat IgG2b Log in to see YFC118-3 3
0.30226976 ABIN118553 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
0.30226976 ABIN118552 FACS FITC Rat IgG2b Log in to see YFC118-3 3
0.30226976 ABIN254640 FACS Biotin Rat IgG2b Log in to see YFC118-3
0.30226976 ABIN2478872 IHC (fro) FACS Rat IgG2b Log in to see YFC118-3 4
0.30226976 ABIN2478853 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9 4
0.0010482287 ABIN319697 FACS PE Rat IgG2b Log in to see YFC118-3 3
0.0010482287 ABIN319696 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 3
0.0010482287 ABIN118554 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
0.0010482287 ABIN182028 FACS FITC Rat IgG2c Log in to see YKIX490-6-4
0.0010482287 ABIN2478878 Func IHC (fro) FACS IP Rat IgG2b Log in to see YFC118-3 4
0.0010482287 ABIN2478876 FACS Biotin Rat IgG2b Log in to see YFC118-3 4
0.0010482287 ABIN2478877 FACS FITC Rat IgG2b Log in to see YFC118-3 4
0.0010482287 ABIN2478874 FACS Alexa Fluor 647 Rat IgG2b Log in to see YFC118-3 4
0.0010482287 ABIN2478879 FACS PE Rat IgG2b Log in to see YFC118-3 4
0.0010482287 ABIN2222121 FACS FITC Rat IgG2c Log in to see 10B561
0.0010482287 ABIN477983 FACS IP Rat IgG2c Log in to see YKIX490-6-4
0.0010482287 ABIN1834920 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9
0.0010482287 ABIN4327005 FACS ICC IF IHC IHC (fro) IP Rat IgG2b Log in to see YFC118-3
0.0010482287 ABIN4327012 FACS IHC IHC (fro) IP DyLight 350 Rat IgG2b Log in to see YFC118-3

Top referenced anti-Integrin beta 2 antibody for Flow Cytometry

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Human Dog (Canine)
Western Blotting (WB) 219 Antibodies
Neutralization (Neut) 3 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunoprecipitation (IP) 133 Antibodies 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 91 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 58 Antibodies 21 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunofluorescence (IF) 43 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 16 Antibodies 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(466), (209), (111), (39), (38), (21), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(363), (186), (155), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is conjugated to Biotin
(92), (77), (52), (19), (14), (11), (9), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(497), (275), (273), (158), (105), (105), (84), (62), (44), (34), (20), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118551) Images
Specificity This antibody recognises the CD18 cell surface antigen, the integrin b2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Purification Affinity chromatography on Protein G.
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Clone YFC118-3
Isotype IgG2b

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118551) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18 is expressed by the majority of leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN118551) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat-1/5 dilutesd antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN118551) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % Bovine Serum Albumin as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 Antibody (ABIN118551)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118551) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 antibody (ITGB2)  (Biotin) (ABIN118551) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD18:BIOTIN