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|Application / Reactivity||Human||Dog (Canine)|
|Western Blotting (WB)||193 Antibodies|
|Neutralization (Neut)||3 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||3 Antibodies|
|Immunoprecipitation (IP)||95 Antibodies||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||40 Antibodies|
|Immunohistochemistry (IHC)||64 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||33 Antibodies||7 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies|
|Immunofluorescence (IF)||38 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Antigen||Integrin beta 2 (ITGB2) Antibodies|
|Reactivity||Dog (Canine), Human Alternatives|
|Conjugate||This Integrin beta 2 antibody is conjugated to PE Alternatives|
Flow Cytometry (FACS)
|3 references available|
|Supplier||Log in to see|
Product Details anti-Integrin beta 2 AntibodyTarget Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118555) Images
|Specificity||YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen as recognising the CD18 antigen. The main cellular reactivity of antibody YFC 118.3 is a strong reactivity with Leucocytes (platelets negative). YFC118.3 blocks homotypic adhesion and other lymphocyte functions which involve the LFA-1 antigen.|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the Y3/Ag.1.2.3 rat myeloma cell line.|
Target Details Integrin beta 2Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118555) Images back to top
|Alternative Name||CD18 / ITGB2 (ITGB2 Antibody Abstract)|
|Background||The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the b chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The CD18 Molecule is the receptor for ICAM-1 and is thus important for cell adhesion.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN118555) Images back to top
Flow cytometry: Use 10 μL of neat antibody to label 100 μL whole blood or 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN118555) Images back to top
|Reconstitution||SM1076R: Restore with 1 mL distilled water. SM1076RT: Restore with 25 μL distilled water.|
|Buffer||PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Aliquot and store antibody and control at -20 °C.|
References for anti-Integrin beta 2 Antibody (ABIN118555)Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Chabanne, Marchal, Kaplanski et al.: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994 (PubMed).
Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988 (PubMed).
Galfrè, Milstein, Wright: "Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG." in: Nature, Vol. 277, Issue 5692, pp. 131-3, 1979 (PubMed).
ImagesProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118555) back to top