anti-Human Integrin beta 2 antibody for Flow Cytometry

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • ITGB2
  • 2E6
  • AI528527
  • Cd18
  • LAD
  • LCAMB
  • Lfa1
  • MF17
  • CD18
  • LFA-1
  • MAC-1
  • MFI7
  • AI256028
  • LTbetaR
  • Ltar
  • TNF-R-III
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFRrp
  • Tnfbr
  • Tnfrsf3
  • D12S370
  • LT-BETA-R
  • TNFR3
  • TNFRSF3
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • integrin, beta 2
  • integrin beta 2
  • lymphotoxin B receptor
  • lymphotoxin beta receptor (TNFR superfamily, member 3)
  • ITGB2
  • Itgb2
  • CD18
  • Ltbr
  • LTBR
Reactivity
Dog (Canine), Human
466
208
118
42
26
21
21
5
4
3
3
3
3
3
2
1
1
1
Host
Rat
342
160
159
10
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is conjugated to PE
85
69
50
19
14
11
9
7
7
7
6
6
6
6
5
5
5
5
5
5
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Flow Cytometry (FACS)
459
284
201
145
95
87
74
67
51
40
26
17
11
9
7
6
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN118555
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.2407675 ABIN118551 FACS Biotin Rat IgG2b Log in to see YFC118-3 2
14.2407675 ABIN118552 FACS FITC Rat IgG2b Log in to see YFC118-3 3
14.2407675 ABIN118553 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
14.2407675 ABIN111901 FACS PE Mouse IgG1 Activation Site Log in to see MEM-148 1
14.2407675 ABIN4896642 FACS Fluorescein Mouse IgG1 AA 23-700 Log in to see 212701 4
14.2407675 ABIN4896643 FACS PE Mouse IgG1 AA 23-700 Log in to see 212701 2
14.2407675 ABIN2478861 FACS IP WB Mouse IgG1 Activation Site Log in to see MEM-148 4
14.2407675 ABIN2478872 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 4
14.2407675 ABIN2144522 FACS FITC Mouse IgG1 Log in to see 4ATS1-18-1-2-11 2
14.2407675 ABIN2144810 FACS PE Mouse IgG1 Log in to see 4ATS1-18-1-2-11 2
14.2407675 ABIN2144808 FACS PE Mouse IgG1 Log in to see 4ATS1-18-1-2-11 2
14.2407675 ABIN2144524 FACS FITC Mouse IgG1 Log in to see 4ATS1-18-1-2-11 2
14.2407675 ABIN2663103 FACS IP IHC WB PE Mouse IgG1 kappa Log in to see TS1-18 7
14.2407675 ABIN2656975 FACS ICFC APC Mouse IgG1 kappa Log in to see TS1-18 6
14.2407675 ABIN2661538 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
14.2407675 ABIN2665569 FACS Mouse IgG1 kappa Log in to see TS1-18 7
14.2407675 ABIN269112 CyTOF FACS Func IP WB Mouse IgG1 Log in to see MEM-148
14.2407675 ABIN2664303 FACS Mouse IgG1 kappa Log in to see TS1-18 6
14.2407675 ABIN254640 FACS Biotin Rat IgG2b Log in to see YFC118-3
14.2407675 ABIN4896641 FACS Allophycocyanin Mouse IgG1 AA 23-700 Log in to see 212701

Top referenced anti-Integrin beta 2 antibody for Flow Cytometry

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Human Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Western Blotting (WB) 221 Antibodies 1 Antibodies
Neutralization (Neut) 3 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunoprecipitation (IP) 104 Antibodies 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 78 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 51 Antibodies 24 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunofluorescence (IF) 50 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(466), (208), (118), (42), (26), (21), (21), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rat
(342), (160), (159), (10), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is conjugated to PE
(85), (69), (50), (19), (14), (11), (9), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS)
(459), (284), (201), (145), (95), (87), (74), (67), (51), (40), (26), (17), (11), (9), (7), (6), (3), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118555) Images
Specificity YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen as recognising the CD18 antigen. The main cellular reactivity of antibody YFC 118.3 is a strong reactivity with Leucocytes (platelets negative). YFC118.3 blocks homotypic adhesion and other lymphocyte functions which involve the LFA-1 antigen.
Purification Affinity chromatography on Protein G
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the Y3/Ag.1.2.3 rat myeloma cell line.
Clone YFC118-3
Isotype IgG2b

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118555) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the b chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The CD18 Molecule is the receptor for ICAM-1 and is thus important for cell adhesion.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118555) Images back to top
Application Notes Flow cytometry: Use 10 μL of neat antibody to label 100 μL whole blood or 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118555) Images back to top
Reconstitution SM1076R: Restore with 1 mL distilled water. SM1076RT: Restore with 25 μL distilled water.
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store antibody and control at -20 °C.

ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118555)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Galfrè, Milstein, Wright: "Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG." in: Nature, Vol. 277, Issue 5692, pp. 131-3, 1979

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118555) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 antibody (ITGB2)  (PE) (ABIN118555) Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18:RPE