anti-Human Integrin beta 2 antibody for Flow Cytometry

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • ITGB2
  • 2E6
  • AI528527
  • Cd18
  • LAD
  • LCAMB
  • Lfa1
  • MF17
  • CD18
  • LFA-1
  • MAC-1
  • MFI7
  • AI256028
  • LTbetaR
  • Ltar
  • TNF-R-III
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFRrp
  • Tnfbr
  • Tnfrsf3
  • D12S370
  • LT-BETA-R
  • TNFR3
  • TNFRSF3
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • integrin, beta 2
  • integrin beta 2
  • lymphotoxin B receptor
  • lymphotoxin beta receptor (TNFR superfamily, member 3)
  • ITGB2
  • Itgb2
  • CD18
  • Ltbr
  • LTBR
Reactivity
Dog (Canine), Human
477
213
118
42
26
21
21
5
4
3
3
3
3
3
2
1
1
1
Host
Rat
352
164
161
10
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is conjugated to Biotin
88
72
51
20
14
11
9
7
7
7
7
6
6
6
5
5
5
5
5
5
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Flow Cytometry (FACS)
472
286
203
147
95
89
75
68
53
40
26
17
11
9
7
6
4
2
1
1
1
1
1
1
1
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Catalog No. ABIN118551
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.320047 ABIN118555 FACS PE Rat IgG2b Log in to see YFC118-3 3
14.320047 ABIN118553 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
14.320047 ABIN118552 FACS FITC Rat IgG2b Log in to see YFC118-3 3
14.320047 ABIN111901 FACS PE Mouse IgG1 Activation Site Log in to see MEM-148 1
14.320047 ABIN2478861 FACS IP WB Mouse IgG1 Activation Site Log in to see MEM-148 4
14.320047 ABIN2478872 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 4
14.320047 ABIN4896643 FACS PE Mouse IgG1 AA 23-700 Log in to see 212701 2
14.320047 ABIN4896642 FACS Fluorescein Mouse IgG1 AA 23-700 Log in to see 212701 4
14.320047 ABIN2665569 FACS Mouse IgG1 kappa Log in to see TS1-18 7
14.320047 ABIN2851784 FACS IF FITC Mouse IgG1 Log in to see
14.320047 ABIN2851674 FACS IF PE Mouse IgG1 Log in to see
14.320047 ABIN2656975 FACS ICFC APC Mouse IgG1 kappa Log in to see TS1-18 6
14.320047 ABIN2661538 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
14.320047 ABIN2663103 FACS IP IHC WB PE Mouse IgG1 kappa Log in to see TS1-18 7
14.320047 ABIN2707998 FACS IF Mouse IgG1 Log in to see
14.320047 ABIN2664303 FACS Mouse IgG1 kappa Log in to see TS1-18 6
14.320047 ABIN269112 CyTOF FACS Func IP WB Mouse IgG1 Log in to see MEM-148
14.320047 ABIN2478853 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9 4
14.320047 ABIN2144522 FACS FITC Mouse IgG1 Log in to see 4ATS1-18-1-2-11 2
14.320047 ABIN2144524 FACS FITC Mouse IgG1 Log in to see 4ATS1-18-1-2-11 2

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(477), (213), (118), (42), (26), (21), (21), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rat
(352), (164), (161), (10), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is conjugated to Biotin
(88), (72), (51), (20), (14), (11), (9), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS)
(472), (286), (203), (147), (95), (89), (75), (68), (53), (40), (26), (17), (11), (9), (7), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118551) Images
Specificity This antibody recognises the CD18 cell surface antigen, the integrin b2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Purification Affinity chromatography on Protein G.
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Clone YFC118-3
Isotype IgG2b

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118551) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18 is expressed by the majority of leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118551) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat-1/5 dilutesd antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118551) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % Bovine Serum Albumin as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118551)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118551) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 (ITGB2) antibody (Biotin) (ABIN118551) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD18:BIOTIN