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anti-Human Integrin beta 2 antibody for Functional Studies

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Epitope
Activation Site
40
32
28
16
14
13
12
8
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
411
200
95
25
24
7
6
3
3
2
2
2
2
2
2
2
1
1
Host
Mouse
281
165
153
5
2
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
70
50
19
9
7
6
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
394
239
183
109
82
64
61
44
39
34
16
9
7
6
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN111902
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000873873 ABIN94005 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48 19
0.000873873 ABIN94010 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 8
0.000873873 ABIN111898 Func FACS IP WB Mouse IgG1 Activation Site Log in to see MEM-148 1
0.000873873 ABIN2749019 Func IHC (f) FACS IP IHC (p) WB Mouse IgG1 Log in to see MEM-48 12
0.000873873 ABIN2749020 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 10
0.000873873 ABIN120700 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.000873873 ABIN298664 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.000873873 ABIN303565 Func FACS IHC (fro) IP WB Biotin Mouse IgG1 Log in to see MEM-48
0.000873873 ABIN462406 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48
0.000873873 ABIN609963 Func FACS FITC Mouse IgG2a Log in to see IB4
0.000873873 ABIN609964 Func FACS PE Mouse IgG2a Log in to see IB4
0.000873873 ABIN609962 Func FACS Biotin Mouse IgG2a Log in to see IB4
0.000873873 ABIN269112 Func FACS IP WB Mouse IgG1 Log in to see MEM-148
0.000873873 ABIN231966 Func FACS IHC (fro) IP WB PE Mouse IgG1 AA 534-546 Log in to see MEM-48
0.000873873 ABIN207018 Func FACS IP WB Mouse IgG1 Log in to see MEM-148
0.000873873 ABIN614281 Func IHC (fro) FACS IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.000873873 ABIN609961 Func FACS Mouse IgG2a Log in to see IB4
0.000873873 ABIN2478878 Func IHC (fro) FACS IP Rat IgG2b Log in to see YFC118-3 4
0.000873873 ABIN1837589 Func IHC (fro) FACS IP WB Mouse IgG1 Log in to see MEM-48
0.000873873 ABIN609965 Func FACS Mouse IgG2a Log in to see IB4

Top referenced anti-Integrin beta 2 antibody for Functional Studies

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
ELISA 74 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 223 Antibodies
Functional Studies (Func) 49 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 63 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 32 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 94 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Intracellular Staining (ICS) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope Activation Site
(40), (32), (28), (16), (14), (13), (12), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(411), (200), (95), (25), (24), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(281), (165), (153), (5), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (70), (50), (19), (9), (7), (6), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(394), (239), (183), (109), (82), (64), (61), (44), (39), (34), (16), (9), (7), (6), (4), (3), (2), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN111902) Images
Specificity The antibody recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin b2 subunit, b2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Cross-Reactivity (Details) Species reactivity (tested):Human. Negative with Porcine.
Purification Protein A Affinity Chromatography
Immunogen Peripheral blood mononuclear cells.
Clone MEM-148
Isotype IgG1

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN111902) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18, integrin b2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN111902) Images back to top
Application Notes Flow Cytometry: The antibody is excellent marker of activated myeloid cells. Immunoprecipitation. Western Blotting (Non Reducing Conditions). Functional Application: The antibody induces homotypic cell aggregation and high-affinityLFA-1 conformation in resting leukocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN111902) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, with 15 mM Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 Antibody (ABIN111902)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Product cited in:

Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009

Background publications

Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007

Peters, Sindrilaru, Wang, Oreshkova, Renkl, Kess, Scharffetter-Kochanek: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006

Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005

Kess, Peters, Zamek, Wickenhauser, Tawadros, Loser, Varga, Grabbe, Nischt, Sunderkötter, Müller, Krieg, Scharffetter-Kochanek: "CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 5697-706, 2003

Drbal, Angelisová, Cerný, Hilgert, Horejsí: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." in: Immunobiology, Vol. 203, Issue 4, pp. 687-98, 2001

Drbal, Angelisová, Hilgert, Cerný, Novák, Horejsí: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, 2001

Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998

Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996