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anti-Human Integrin beta 2 antibody for Immunofluorescence

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Epitope
pThr758
40
31
28
25
16
14
13
9
8
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
466
208
110
39
39
21
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rabbit
363
187
154
5
3
1
Clonality
Polyclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
92
77
53
19
14
11
9
8
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
498
274
273
158
105
105
83
62
43
34
20
9
7
6
5
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1531540
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN1532556 IF ELISA Rabbit IgG Log in to see Polyclonal 2
0.32040194 ABIN1105953 EIA IF Mouse IgG2b Log in to see 1-00E-013 3
0.32040194 ABIN933062 IF ELISA Mouse IgG2b Log in to see 1E13
0.32040194 ABIN2490960 IF ELISA WB Rabbit IgG pThr758 Log in to see Polyclonal
0.32040194 ABIN2490959 IF ELISA Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN2661538 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
0.0010840179 ABIN2665956 IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see TS1-18 6
0.0010840179 ABIN466714 IF ELISA Mouse IgG2b Log in to see 10E12
0.0010840179 ABIN189488 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser745 Log in to see EP1288Y
0.0010840179 ABIN2888794 IF ELISA Rabbit IgG AA 720-769 Log in to see Polyclonal
0.0010840179 ABIN2661539 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
0.0010840179 ABIN2851674 FACS IF PE Mouse IgG1 Log in to see
0.0010840179 ABIN2851784 FACS IF FITC Mouse IgG1 Log in to see
0.0010840179 ABIN2707998 FACS IF Mouse IgG1 Log in to see
0.0010840179 ABIN1112070 FACS IF PE Mouse IgG1 Log in to see GRF1
0.0010840179 ABIN3183040 ELISA IF Rabbit IgG pThr758 Log in to see Polyclonal
0.0010840179 ABIN3185203 ELISA IF Rabbit IgG Ser63 Log in to see Polyclonal
0.0010840179 ABIN2869416 IF Mouse IgG2b Log in to see
0.0010840179 ABIN2478853 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9 4
0.0010840179 ABIN1583608 IF Mouse IgG2b Log in to see 1E-13

Top referenced anti-Integrin beta 2 antibody for Immunofluorescence

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 6 Antibodies
ELISA 14 Antibodies 21 Antibodies
Flow Cytometry (FACS) 41 Antibodies 126 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Western Blotting (WB) 30 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope pThr758
(40), (31), (28), (25), (16), (14), (13), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(466), (208), (110), (39), (39), (21), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(363), (187), (154), (5), (3), (1)
Clonality
Polyclonal
Conjugate This Integrin beta 2 antibody is un-conjugated
(92), (77), (53), (19), (14), (11), (9), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(498), (274), (273), (158), (105), (105), (83), (62), (43), (34), (20), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) Images
Specificity CD18/ITGB2 (Phospho-Thr758) Antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at Thr758.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758.
Isotype IgG

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) Images back to top
Antigen
Alternative Name CD18/ITGB2 (ITGB2 Antibody Abstract)
Background Molecular weight of target protein: 84 kDa
Synonyms: CD18, cell surface adhesion glycoproteins LFA- 1/CR3/p150,95 beta, complement receptor C3 beta, integrin beta-2, ITB2
Molecular Weight 84 kDa
Gene ID 3689
OMIM 116920
UniProt P05107
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.375957 (NCBI Gene Symbol: ITGB2)

Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN1531540) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-Integrin beta 2 Antibody (ABIN1531540)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Hattori, Fujiyama, Taylor, Watanabe, Yada, Park, Toyoda, Ishii, Totoki, Choi, Groner, Soeda, Ohki, Takagi, Sakaki, Taudien, Blechschmidt, Polley, Menzel, Delabar, Kumpf, Lehmann, Patterson, Reichwald et al.: "The DNA sequence of human chromosome 21. ..." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000

Kishimoto, OConnor, Lee, Roberts, Springer: "Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family." in: Cell, Vol. 48, Issue 4, pp. 681-90, 1987

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1531540) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (pThr758) (ABIN1531540) Western blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30', usin...
Immunofluorescence (IF) image for anti-Integrin beta 2 antibody (ITGB2) (pThr758) (ABIN1531540) Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibod...