anti-Human Integrin beta 2 antibody for Immunofluorescence

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • ITGB2
  • 2E6
  • AI528527
  • Cd18
  • LAD
  • LCAMB
  • Lfa1
  • MF17
  • CD18
  • LFA-1
  • MAC-1
  • MFI7
  • AI256028
  • LTbetaR
  • Ltar
  • TNF-R-III
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFRrp
  • Tnfbr
  • Tnfrsf3
  • D12S370
  • LT-BETA-R
  • TNFR3
  • TNFRSF3
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • integrin, beta 2
  • integrin beta 2
  • lymphotoxin B receptor
  • lymphotoxin beta receptor (TNFR superfamily, member 3)
  • ITGB2
  • Itgb2
  • CD18
  • Ltbr
  • LTBR
Epitope
pThr758
46
34
26
23
16
15
14
14
12
5
5
5
5
5
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
476
212
117
43
26
21
21
5
4
3
3
3
3
3
2
1
1
1
Host
Rabbit
351
165
160
10
3
1
Clonality
Polyclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
88
72
52
20
14
11
9
7
7
7
7
6
6
6
5
5
5
5
5
5
3
3
3
3
3
3
3
3
2
2
2
2
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
472
285
202
146
95
88
75
68
52
40
26
17
11
9
7
6
3
2
1
1
1
1
1
1
Options
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Catalog No. ABIN1531540
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.326117 ABIN1532556 IF ELISA Rabbit IgG Log in to see Polyclonal 2
12.326117 ABIN1105953 EIA IF Mouse IgG2b Log in to see 1-00E-013 3
12.326117 ABIN4327005 FACS ICC IF IHC IHC (fro) IP Rat IgG2b Log in to see YFC118-3
12.326117 ABIN4327007 ELISA ICC IF Mouse IgG2b Log in to see 10E12
12.326117 ABIN933062 IF ELISA Mouse IgG2b Log in to see 1E13
1 ABIN2661538 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
1 ABIN2665956 IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see TS1-18 6
1 ABIN466714 IF ELISA Mouse IgG2b Log in to see 10E12
1 ABIN2888794 IF ELISA Rabbit IgG AA 720-769 Log in to see Polyclonal
1 ABIN2478853 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9 4
1 ABIN207172 FACS IF Biotin Mouse IgG1 AA 534-546 Log in to see MEM-48
1 ABIN2851674 FACS IF PE Mouse IgG1 Log in to see
1 ABIN2851784 FACS IF FITC Mouse IgG1 Log in to see
1 ABIN2661539 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
1 ABIN2707998 FACS IF Mouse IgG1 Log in to see
1 ABIN3185203 ELISA IF Rabbit IgG Ser63 Log in to see Polyclonal
1 ABIN3183040 ELISA IF Rabbit IgG pThr758 Log in to see Polyclonal
1 ABIN1112070 FACS IF PE Mouse IgG1 Log in to see GRF1
1 ABIN4327081 FACS IF Mouse IgG2a kappa Log in to see 68-5A5
1 ABIN4327083 FACS IF Mouse IgG2a kappa Log in to see 68-5A5

Top referenced anti-Integrin beta 2 antibody for Immunofluorescence

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 9 Antibodies
ELISA 15 Antibodies 25 Antibodies
Flow Cytometry (FACS) 37 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Western Blotting (WB) 35 Antibodies
Activity Inhibition (Act Inh) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope pThr758
(46), (34), (26), (23), (16), (15), (14), (14), (12), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(476), (212), (117), (43), (26), (21), (21), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(351), (165), (160), (10), (3), (1)
Clonality
Polyclonal
Conjugate This Integrin beta 2 antibody is un-conjugated
(88), (72), (52), (20), (14), (11), (9), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(472), (285), (202), (146), (95), (88), (75), (68), (52), (40), (26), (17), (11), (9), (7), (6), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN1531540) Images
Specificity CD18/ITGB2 (Phospho-Thr758) Antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at Thr758.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CD18/ITGB2 around the phosphorylation site of Thr758.
Isotype IgG

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Antigen
Alternative Name CD18/ITGB2 (ITGB2 Antibody Abstract)
Background Molecular weight of target protein: 84 kDa
Synonyms: CD18, cell surface adhesion glycoproteins LFA- 1/CR3/p150,95 beta, complement receptor C3 beta, integrin beta-2, ITB2
Molecular Weight 84 kDa
Gene ID 3689
OMIM 116920
UniProt P05107
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.375957 (NCBI Gene Symbol: ITGB2)

Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details ProductDetails: References for anti-Integrin beta 2 antibody (ABIN1531540) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-Integrin beta 2 antibody (ABIN1531540)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Hattori, Fujiyama, Taylor, Watanabe, Yada, Park, Toyoda, Ishii, Totoki, Choi, Groner, Soeda, Ohki, Takagi, Sakaki, Taudien, Blechschmidt, Polley, Menzel, Delabar, Kumpf, Lehmann, Patterson, Reichwald et al.: "The DNA sequence of human chromosome 21. ..." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000

Kishimoto, OConnor, Lee, Roberts, Springer: "Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family." in: Cell, Vol. 48, Issue 4, pp. 681-90, 1987

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN1531540) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (pThr758) (ABIN1531540) Western blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30', usin...
Immunofluorescence (IF) image for anti-Integrin beta 2 antibody (ITGB2) (pThr758) (ABIN1531540) Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) Antibod...