You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Integrin beta 2 antibody for Immunohistochemistry

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Epitope
Ser745
40
32
28
25
16
14
13
9
8
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
465
204
109
39
38
20
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rabbit
360
181
155
5
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
76
51
19
14
11
9
7
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
489
269
266
152
104
100
83
58
44
34
18
9
7
6
5
3
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN189488
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22874774 ABIN2469881 FACS IP IHC WB Mouse IgG1 AA 534-546 Log in to see MEM-48
0.22874774 ABIN2470788 FACS IP IHC WB Mouse IgG1 AA 534-546 Log in to see MEM-48
0.22874774 ABIN1584185 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.22874774 ABIN2423669 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0011326512 ABIN2661538 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
0.0011326512 ABIN2665956 IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see TS1-18 6
0.0011326512 ABIN2663103 FACS IP IHC WB PE Mouse IgG1 kappa Log in to see TS1-18 7
0.0011326512 ABIN1873315 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011326512 ABIN2661539 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
0.0011326512 ABIN2663104 FACS IP IHC WB PE Mouse IgG1 kappa Log in to see TS1-18 7
0.0011326512 ABIN438290 FACS IHC (fro) IP IHC WB DyLight 488 Mouse IgG1 Log in to see MEM-48
0.0011326512 ABIN3023118 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011326512 ABIN3185341 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011326512 ABIN600733 FACS IP IHC WB Mouse IgG1 Log in to see
0.0011326512 ABIN269011 Func FACS IHC (fro) IHC (p) IP IHC WB Mouse IgG1 AA 534-546 Log in to see MEM-48
0.0011326512 ABIN2878092 IHC WB Rabbit Log in to see Polyclonal
0.0011326512 ABIN345377 FACS IHC WB Mouse IgG1 Log in to see MEM-48
0.0011326512 ABIN2292351 IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.0011326512 ABIN2292395 IHC WB Rabbit AA 24-58 Log in to see Polyclonal
0.0011326512 ABIN2292397 IHC WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 6 Antibodies
ELISA 14 Antibodies 20 Antibodies
Flow Cytometry (FACS) 41 Antibodies 126 Antibodies
Functional Studies (Func) 1 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 2 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Western Blotting (WB) 30 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Epitope Ser745
(40), (32), (28), (25), (16), (14), (13), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(465), (204), (109), (39), (38), (20), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(360), (181), (155), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (76), (51), (19), (14), (11), (9), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(489), (269), (266), (152), (104), (100), (83), (58), (44), (34), (18), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling Images
Specificity The antibody only detects LFA-Beta2 phosphorylated on Serine 745.
Purification Tissue culture supernatant
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser745 of human LFA-Beta2 was used as immunogen.
Clone EP1288Y
Isotype IgG

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background In the immune system, integrins have essential roles in leukocyte trafficking andfunction. These include immune cell attachment to endothelial and antigen-presentingcells, cytotoxicity, and extravasation into tissues (1). Integrin adhesion receptorstransduce signals that control complex cell functions which require the regulation ofgene expression, such as proliferation, differentiation and survival. Their intracellulardomain has no catalytic function, indicating that interaction with other transducingmolecules is crucial for integrin-mediated signaling. JAB1 (Jun activationdomain-binding protein 1), a coactivator of the c-Jun transcription factor, has beenidentified as a protein that interacts with the cytoplasmic domain of the beta2 subunit ofthe alphaL/beta2 integrin LFA-1 (2). Patients with leukocyte adhesion molecule(CD11/CD18, beta 2 integrins) deficiency have structural defects in the common betasubunit (CD18), which prevent heterodimer formation and normal cell surfaceexpression of these receptors, leading to life-threatening bacterial infections (3). Alternate Names: anti-Beta 2 (antigen CD18 (p95) antibody, anti-CD 18 antibody, anti-CD18 antigenantibody, anti-Cell surface adhesion glycoproteins LFA 1/CR3/p150 95 subunit betaantibody, anti-Cell surface adhesion glycoproteins LFA 1/CR3/p15095 beta subunitantibody, anti-Complement component 3 receptor 3 and 4 subunit antibody,anti-Complement receptor C3 beta subunit antibody, anti-Integrin beta 2 antibody,anti-Integrin beta chain beta 2 antibody, anti-ITGB 2 antibody, anti-ITGB2 antibody,anti-LAD antibody, anti-LCAMB antibody, anti-Leukocyte Cell Adhesion Molecule CD18antibody, anti-LFA 1 antibody, anti-LFA1 antibody, anti-Lymphocyte function associatedantigen 1 antibody, anti-MAC 1 antibody, anti-MAC1 antibody, anti-Macrophage antigen1 (mac1) beta subunit antibody, anti-MF17 antibody, anti-MFI7 antibody.
Gene Symbol: ITGB2
Gene ID 3689, 16414, 309684
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 85kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:100, Western Blot 1:1000-10000
Protocol Protocol specific for Integrin beta 2/CD18 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (Ser745) (ABIN189488) Western blot analysis on Jurkat cell lysates using anti-Phospho-LFA-?2 (pS745) RabMAb...
Immunohistochemistry (IHC) image for anti-Integrin beta 2 antibody (ITGB2) (Ser745) (ABIN189488) Immunohistochemical analysis of human tonsils using anti-Phospho- LFA-?2 (pS745) RabM...