|Application / Reactivity||Human|
|Blocking Reagent (BR)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||223 Antibodies|
|Functional Studies (Func)||50 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies|
|Immunohistochemistry (IHC)||64 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||33 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||40 Antibodies|
|Immunoprecipitation (IP)||95 Antibodies|
|Intracellular Flow Cytometry (ICFC)||3 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Antigen||Integrin beta 2 (ITGB2) Antibodies|
|Conjugate||This Integrin beta 2 antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|12 references available|
|Supplier||Log in to see|
Product Details anti-Integrin beta 2 AntibodyTarget Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) Images
|Specificity||The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.|
|No Cross-Reactivity||Dog (Canine)|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.|
|Immunogen||Leukocytes of a patient suffering from a LGL-type leukemia.|
Target Details Integrin beta 2Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) Images back to top
|Alternative Name||CD18 (ITGB2 Antibody Abstract)|
|Background||CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.|
|Research Area||Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN2749019) Images back to top
|Buffer||Azide free phosphate buffered saline (PBS), approx. pH 7.4|
|Storage Comment||Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.|
References for anti-Integrin beta 2 Antibody (ABIN2749019)Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
|Product cited in:||
Kuttruff, Koch, Kelp et al.: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009 (PubMed).
Garnotel, Rittié, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).
Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, 1990 (PubMed).
Bazil, Stefanová, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein." in: Folia biologica, Vol. 36, Issue 1, pp. 41-50, 1990 (PubMed).
Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991 (PubMed).
Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007 (PubMed).
Peters, Sindrilaru, Wang et al.: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006 (PubMed).
Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005 (PubMed).
Kess, Peters, Zamek et al.: "CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 5697-706, 2003 (PubMed).
Drbal, Angelisová, Cerný et al.: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, 2000 (PubMed).
Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998 (PubMed).
Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996 (PubMed).
ImagesProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) back to top