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anti-Human Integrin beta 2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Reactivity
Human
411
200
95
25
24
7
6
3
3
2
2
2
2
2
2
2
1
1
Host
Mouse
281
165
153
5
2
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
70
50
19
9
7
6
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
394
239
183
109
82
64
61
43
39
34
16
9
7
6
4
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2749019
$ 231.00
Plus shipping costs $45.00

Top referenced anti-Integrin beta 2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
ELISA 74 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 223 Antibodies
Functional Studies (Func) 49 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 63 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 32 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 94 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Intracellular Staining (ICS) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Human
(411), (200), (95), (25), (24), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(281), (165), (153), (5), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (70), (50), (19), (9), (7), (6), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(394), (239), (183), (109), (82), (64), (61), (43), (39), (34), (16), (9), (7), (6), (4), (2), (2), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) Images
Specificity The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
No Cross-Reactivity Dog (Canine)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Immunogen Leukocytes of a patient suffering from a LGL-type leukemia.
Clone MEM-48
Isotype IgG1

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) Images back to top
Antigen
Alternative Name CD18 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Research Area Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN2749019) Images back to top
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4
Preservative Azide free
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-Integrin beta 2 Antibody (ABIN2749019)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Product cited in:

Kuttruff, Koch, Kelp, Pawelec, Rammensee, Steinle: "NKp80 defines and stimulates a reactive subset of CD8 T cells." in: Blood, Vol. 113, Issue 2, pp. 358-69, 2009

Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000

Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Horejsí: "Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein." in: Folia biologica, Vol. 36, Issue 1, pp. 41-50, 1990

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, 1990

Background publications

Dassanayake, Maheswaran, Srikumaran: "Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects." in: Infection and immunity, Vol. 75, Issue 10, pp. 5004-10, 2007

Peters, Sindrilaru, Wang, Oreshkova, Renkl, Kess, Scharffetter-Kochanek: "CD18 in monogenic and polygenic inflammatory processes of the skin." in: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 11, Issue 1, pp. 7-15, 2006

Solovjov, Pluskota, Plow: "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1336-45, 2005

Kess, Peters, Zamek, Wickenhauser, Tawadros, Loser, Varga, Grabbe, Nischt, Sunderkötter, Müller, Krieg, Scharffetter-Kochanek: "CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 11, pp. 5697-706, 2003

Drbal, Angelisová, Cerný, Pavlistová, Cebecauer, Novák, Horejsí: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, 2000

Shang, Issekutz: "Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers." in: European journal of immunology, Vol. 28, Issue 6, pp. 1970-9, 1998

Gao, Issekutz: "Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers." in: Immunology, Vol. 88, Issue 3, pp. 463-70, 1996

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN2749019) back to top
Supplier Images
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN2749019) Fig. A Flow Cytometry analysis. Surface staining of human peripheral blood cells with...
Western Blotting (WB) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN2749019) Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-h...
Immunohistochemistry (IHC) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN2749019) Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Co...