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anti-Human Integrin beta 2 antibody for Immunohistochemistry (Frozen Sections)

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Reactivity
Dog (Canine), Human
466
209
111
39
38
21
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rat
363
186
155
5
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
92
77
53
19
14
11
9
8
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
497
275
272
158
105
104
84
62
44
34
20
9
7
6
5
3
2
1
1
1
1
1
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Catalog No. ABIN118553
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN319696 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 3
0.000883884 ABIN94005 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48 19
0.000883884 ABIN118554 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
0.000883884 ABIN111897 FACS IHC (fro) IP WB Mouse IgG1 Activation Site Log in to see MEM-148
0.000883884 ABIN120700 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.000883884 ABIN298664 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.000883884 ABIN303565 Func FACS IHC (fro) IP WB Biotin Mouse IgG1 Log in to see MEM-48
0.000883884 ABIN462406 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48
0.000883884 ABIN284136 FACS IHC (fro) IP WB Mouse IgG1 Log in to see MEM48
0.000883884 ABIN2478872 IHC (fro) FACS Rat IgG2b Log in to see YFC118-3 4
0.000883884 ABIN438290 FACS IHC (fro) IP IHC WB DyLight 488 Mouse IgG1 Log in to see MEM-48
0.000883884 ABIN231966 Func FACS IHC (fro) IP WB PE Mouse IgG1 AA 534-546 Log in to see MEM-48
0.000883884 ABIN206736 BR FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3
0.000883884 ABIN614281 Func IHC (fro) FACS IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.000883884 ABIN1859504 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 124-363 Log in to see Polyclonal
0.000883884 ABIN1859503 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 451-627 Log in to see Polyclonal
0.000883884 ABIN600732 IHC (fro) FACS IP Rat IgG2b Log in to see
0.000883884 ABIN1723359 IHC (fro) FACS IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.000883884 ABIN2478878 Func IHC (fro) FACS IP Rat IgG2b Log in to see YFC118-3 4
0.000883884 ABIN1837589 Func IHC (fro) FACS IP WB Mouse IgG1 Log in to see MEM-48

Top referenced anti-Integrin beta 2 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
ELISA 75 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 273 Antibodies
Functional Studies (Func) 74 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 43 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 91 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 58 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 133 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Intracellular Staining (ICS) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(466), (209), (111), (39), (38), (21), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(363), (186), (155), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(92), (77), (53), (19), (14), (11), (9), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(497), (275), (272), (158), (105), (104), (84), (62), (44), (34), (20), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118553) Images
Specificity This antibody recognises the CD18 cell surface antigen, the integrin b2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Purification Purified
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Clone YFC118-3
Isotype IgG2b

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118553) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18 is expressed by the majority of leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN118553) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/1000 - 1/5000. Recommended positive control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN118553) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 Antibody (ABIN118553)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118553) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN118553) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD18