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anti-Human Integrin beta 2 antibody for Immunoprecipitation

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Reactivity
Dog (Canine), Human
464
204
109
39
37
20
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rat
360
180
155
5
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
76
51
19
14
11
9
7
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
488
269
265
152
104
99
83
58
44
34
18
9
7
6
5
3
2
1
1
1
1
1
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Catalog No. ABIN118553
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089579594 ABIN111902 Func FACS IP WB Mouse IgG1 Activation Site Log in to see MEM-148 9
0.00089579594 ABIN319696 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 3
0.00089579594 ABIN94005 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48 19
0.00089579594 ABIN118554 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
0.00089579594 ABIN111897 FACS IHC (fro) IP WB Mouse IgG1 Activation Site Log in to see MEM-148
0.00089579594 ABIN94010 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 8
0.00089579594 ABIN111898 Func FACS IP WB Mouse IgG1 Activation Site Log in to see MEM-148 1
0.00089579594 ABIN2661538 ICC FACS IF IP IHC WB FITC Mouse IgG1 kappa Log in to see TS1-18 6
0.00089579594 ABIN2665956 IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see TS1-18 6
0.00089579594 ABIN2663103 FACS IP IHC WB PE Mouse IgG1 kappa Log in to see TS1-18 7
0.00089579594 ABIN2749019 Func IHC (f) FACS IP IHC (p) WB Mouse IgG1 Log in to see MEM-48 12
0.00089579594 ABIN2749020 Func FACS IP WB Mouse IgG1 Log in to see MEM-148 10
0.00089579594 ABIN189488 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser745 Log in to see EP1288Y
0.00089579594 ABIN120700 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.00089579594 ABIN2469881 FACS IP IHC WB Mouse IgG1 AA 534-546 Log in to see MEM-48
0.00089579594 ABIN298664 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see MEM-48
0.00089579594 ABIN303565 Func FACS IHC (fro) IP WB Biotin Mouse IgG1 Log in to see MEM-48
0.00089579594 ABIN462406 Func FACS IHC (fro) IHC (p) IP WB Mouse IgG1 AA 534-546 Log in to see MEM-48
0.00089579594 ABIN211663 FACS IP WB Mouse IgG1 Activation Site Log in to see
0.00089579594 ABIN2470788 FACS IP IHC WB Mouse IgG1 AA 534-546 Log in to see MEM-48

Top referenced anti-Integrin beta 2 antibody for Immunoprecipitation

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
ELISA 75 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 273 Antibodies
Functional Studies (Func) 74 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 43 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 91 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 58 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 133 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Intracellular Staining (ICS) 1 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(464), (204), (109), (39), (37), (20), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(360), (180), (155), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (76), (51), (19), (14), (11), (9), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(488), (269), (265), (152), (104), (99), (83), (58), (44), (34), (18), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118553) Images
Specificity This antibody recognises the CD18 cell surface antigen, the integrin b2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Purification Purified
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Clone YFC118-3
Isotype IgG2b

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118553) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18 is expressed by the majority of leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN118553) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/1000 - 1/5000. Recommended positive control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN118553) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 Antibody (ABIN118553)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN118553) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN118553) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD18