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anti-Mouse (Murine) Integrin beta 2 antibody for Flow Cytometry

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Reactivity
Mouse (Murine)
465
203
109
39
38
20
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rat
360
180
155
5
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
76
51
19
14
11
9
7
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
488
268
265
152
103
99
83
57
44
34
18
9
7
6
5
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN457404
$ 219.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30379575 ABIN457348 FACS FITC Rat IgG2a Log in to see M18-2 9
0.30379575 ABIN457430 Func FACS IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see M18-2 8
0.30379575 ABIN2657653 FACS Alexa Fluor 647 Rat IgG2a kappa Log in to see M18-2 9
0.30379575 ABIN2656819 FACS Alexa Fluor 594 Rat IgG2a kappa Log in to see M18-2 8
0.30379575 ABIN2663105 BR FACS PE Rat IgG2a kappa Log in to see M18-2 9
0.30379575 ABIN439881 FACS IHC (fro) IHC (p) IP IHC WB Biotin Rat IgG2a kappa Log in to see C71-16
0.30379575 ABIN439882 FACS IHC (fro) IHC (p) IP IHC WB PE Rat IgG2a kappa Log in to see C71-16
0.30379575 ABIN439880 FACS IHC (fro) IHC (p) IP IHC WB FITC Rat IgG2a kappa Log in to see C71-16
0.30379575 ABIN2664306 FACS Rat IgG2a kappa Log in to see M18-2 9
0.30379575 ABIN262325 FACS IHC (fro) IHC (p) IP IHC WB Rat IgG2a kappa Log in to see M18-2
0.30379575 ABIN2490022 FACS FITC Rat IgG2a Log in to see
0.30379575 ABIN2490026 FACS IP WB Rat IgG2a Log in to see
0.30379575 ABIN2490027 FACS IP WB Rat IgG2a Log in to see
0.30379575 ABIN2490028 FACS IP WB Rat IgG2a Log in to see
0.30379575 ABIN2168654 FACS Biotin Rat IgG2a Log in to see C71-16
0.30379575 ABIN2168657 FACS PE Rat IgG2a Log in to see C71-16
0.30379575 ABIN2168655 FACS FITC Rat IgG2a Log in to see C71-16
0.30379575 ABIN2168658 FACS Rat IgG2a Log in to see C71-16
0.30379575 ABIN2168656 FACS Rat IgG2a Log in to see C71-16
0.0010485112 ABIN118151 FACS IHC (fro) IHC (p) Rat IgG2a Log in to see C71-16 5

Top referenced anti-Integrin beta 2 antibody for Flow Cytometry

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 6 Antibodies
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 126 Antibodies
Functional Studies (Func) 17 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 60 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 76 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 2 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Mouse (Murine)
(465), (203), (109), (39), (38), (20), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(360), (180), (155), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (76), (51), (19), (14), (11), (9), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(488), (268), (265), (152), (103), (99), (83), (57), (44), (34), (18), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN457404) Images
Specificity The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit, beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
No Cross-Reactivity Dog (Canine)
Purification Purified by protein G
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Murine cytotoxic T cell glycoproteins
Clone M18-2
Isotype IgG2a

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN457404) Images back to top
Antigen
Alternative Name ITGB2 (ITGB2 Antibody Abstract)
Background CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts.
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN457404) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 1 µg/mL
Functional Application: Blocking or stimulation

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN457404) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-Integrin beta 2 Antibody (ABIN457404)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Product cited in:

Cullere, Lauterbach, Tsuboi, Mayadas: "Neutrophil-selective CD18 silencing using RNA interference in vivo." in: Blood, Vol. 111, Issue 7, pp. 3591-8, 2008

Varga, Balkow, Wild, Stadtbaeumer, Krummen, Rothoeft, Higuchi, Beissert, Wethmar, Scharffetter-Kochanek, Vestweber, Grabbe: "Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation." in: Blood, Vol. 109, Issue 2, pp. 661-9, 2007

Watts, Beurskens, Martin-Padura, Ballantyne, Klickstein, Brenner, Lee: "Manifestations of inflammatory arthritis are critically dependent on LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 6, pp. 3668-75, 2005

Barlow, Langston, Matthews, Chidlow, Kevil: "CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes." in: The American journal of pathology, Vol. 165, Issue 6, pp. 1849-52, 2004

Sakurai, Taguchi, Anand, Ambati, Gragoudas, Miller, Adamis, Ambati: "Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization." in: Investigative ophthalmology & visual science, Vol. 44, Issue 6, pp. 2743-9, 2003

Abraham, Miller: "Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5193-201, 2001

Avni, Pur, Yefenof, Baniyash: "Complement receptor 3 of macrophages is associated with galectin-1-like protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6151-8, 1998

Zahalka, Naor: "Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness." in: International immunology, Vol. 6, Issue 6, pp. 917-24, 1994

Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, 1993

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN457404) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 antibody (ITGB2) (ABIN457404) Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).