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anti-Mouse (Murine) Integrin beta 2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Reactivity
Mouse (Murine)
465
203
109
39
38
20
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rat
360
180
155
5
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
90
76
51
19
14
11
9
7
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Blocking Reagent (BR), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
488
269
266
152
103
99
83
58
44
34
18
8
7
6
5
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1176975
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008711731 ABIN2689033 BR IHC (f) IHC (fro) FACS IP WB Rat IgG2a, kappa Log in to see M18-2 7

Top referenced anti-Integrin beta 2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 6 Antibodies
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 126 Antibodies
Functional Studies (Func) 17 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 60 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 76 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 2 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Mouse (Murine)
(465), (203), (109), (39), (38), (20), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(360), (180), (155), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(90), (76), (51), (19), (14), (11), (9), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Blocking Reagent (BR), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(488), (269), (266), (152), (103), (99), (83), (58), (44), (34), (18), (8), (7), (6), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1176975) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse CTL glycoproteins
Clone M18-2
Isotype IgG2a, kappa

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1176975) Images back to top
Antigen
Alternative Name CD18 (ITGB2 Antibody Abstract)
Background The M18/2 antibody reacts with the common beta2 chain of LFA-1 (CD11a/CD18, alphaLbeta2 integrin), Mac-1 (CD11b/CD18, alphaMbeta2 integrin), and gp150, 95 (CD11c/CD18, alphaXbeta2 integrin). Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin alpha chains (CD11a, CD11b, and CD11c). Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. The beta2 integrins are important mediators of leukocyte-endothelium interactions. It has been reported that M18/2 antibody blocks in vivo metastasis of the LB lymphoma to the spleen and that it blocks in vitro formation of aggregates of LB cells and splenocytes. However, other reports indicate that mAb M18/2 has no effect on CTLmediated killing, adherence of C3bi-sensitized erythrocytes to Mac-1, antigen-specific binding of T cells to antigen-producing cells, or rejection of cardiac allografts. Recent in vitro studies indicate that M18/2 antibody stimulates adhesion of Mac-1 to its ligands C3bi and ICAM-1, and it stimulates adhesion of LFA-1 to ICAM-1, but it has no effect upon the interactions of LFA-1 with ICAM-2 nor ICAM-3.
Synonyms: Integrin beta2 chain
Research Area Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN1176975) Images back to top
Application Notes The alternative anti-CD18 mAb GAME-46 (Cat. No. 555280) is reported to block in vitro adhesion of LFA-1-expressing cells to the ligands ICAM-1, ICAM-2, and ICAM-3 and of Mac-1-expressing cells to ICAM-1, C3bi, and fibrinogen.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN1176975) Images back to top
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-Integrin beta 2 Antibody (ABIN1176975)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Product cited in:

Driessens, van Hulten, Zuurbier, La Rivière, Roos: "Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct." in: Journal of leukocyte biology, Vol. 60, Issue 6, pp. 758-65, 1997

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994

Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, 1993

Isobe, Yagita, Okumura, Ihara: "Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1." in: Science (New York, N.Y.), Vol. 255, Issue 5048, pp. 1125-7, 1992

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983