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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||6 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||123 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||14 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies|
|Immunohistochemistry (IHC)||58 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||2 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||25 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies|
|Immunoprecipitation (IP)||73 Antibodies|
|Intracellular Flow Cytometry (ICFC)||3 Antibodies|
|Mass Cytometry (CyTOF)||1 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Western Blotting (WB)||76 Antibodies|
|Antigen||Integrin beta 2 (ITGB2) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This Integrin beta 2 antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
|6 references available|
|Supplier||Log in to see|
Product Details anti-Integrin beta 2 AntibodyTarget Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1176975) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||Mouse CTL glycoproteins|
Target Details Integrin beta 2Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN1176975) Images back to top
|Alternative Name||CD18 (ITGB2 Antibody Abstract)|
The M18/2 antibody reacts with the common beta2 chain of LFA-1 (CD11a/CD18, alphaLbeta2 integrin), Mac-1 (CD11b/CD18, alphaMbeta2 integrin), and gp150, 95 (CD11c/CD18, alphaXbeta2 integrin). Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin alpha chains (CD11a, CD11b, and CD11c). Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. The beta2 integrins are important mediators of leukocyte-endothelium interactions. It has been reported that M18/2 antibody blocks in vivo metastasis of the LB lymphoma to the spleen and that it blocks in vitro formation of aggregates of LB cells and splenocytes. However, other reports indicate that mAb M18/2 has no effect on CTLmediated killing, adherence of C3bi-sensitized erythrocytes to Mac-1, antigen-specific binding of T cells to antigen-producing cells, or rejection of cardiac allografts. Recent in vitro studies indicate that M18/2 antibody stimulates adhesion of Mac-1 to its ligands C3bi and ICAM-1, and it stimulates adhesion of LFA-1 to ICAM-1, but it has no effect upon the interactions of LFA-1 with ICAM-2 nor ICAM-3.
Synonyms: Integrin beta2 chain
|Research Area||Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN1176975) Images back to top
|Application Notes||The alternative anti-CD18 mAb GAME-46 (Cat. No. 555280) is reported to block in vitro adhesion of LFA-1-expressing cells to the ligands ICAM-1, ICAM-2, and ICAM-3 and of Mac-1-expressing cells to ICAM-1, C3bi, and fibrinogen.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN1176975) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-Integrin beta 2 Antibody (ABIN1176975)Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
|Product cited in:||
Driessens, van Hulten, Zuurbier et al.: "Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct." in: Journal of leukocyte biology, Vol. 60, Issue 6, pp. 758-65, 1997 (PubMed).
Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, 1993 (PubMed).
Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).
Sanchez-Madrid, Simon, Thompson et al.: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983 (PubMed).
Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).
Isobe, Yagita, Okumura et al.: "Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1." in: Science (New York, N.Y.), Vol. 255, Issue 5048, pp. 1125-7, 1992 (PubMed).