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|Antigen||Integrin beta 2 (ITGB2) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This Integrin beta 2 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|5 references available|
|Supplier||Log in to see|
Product Details anti-Integrin beta 2 AntibodyTarget Details Integrin beta 2 Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118151) Images
|Specificity||This antibody is specific for the common β2subunit of LFA-1 (CD11a/CD18), MAC-1 (CD11b/CD18) and P150,95 (CD11c/CD18) (2,3). These three β2 integrins, which function in cell-cell adhesion in the immune system, are also known as leukocyte integrins because their expression is limited to leukocytes (2,3).|
|Immunogen||Cell membrane glycoproteins derived from BW5147 cells (1)|
Target Details Integrin beta 2Product Details anti-Integrin beta 2 Antibody Application Details Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118151) Images back to top
|Alternative Name||CD18 / ITGB2 (ITGB2 Antibody Abstract)|
|Background||CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation|
Application DetailsProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118151) Images back to top
IHC on acetone-fixed frozen sections. Immunoprecipitation. Flow cytometry (please see protocol for details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118151) Images back to top
|Buffer||PBS and 0.02 % sodium azide (NaN3)|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. Dilute only prior to immediate use|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ProductDetails: References for anti-Integrin beta 2 antibody (ABIN118151)Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994
Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, 1990
Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990
Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, 1983
Trowbridge, Omary: "Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1." in: The Journal of experimental medicine, Vol. 154, Issue 5, pp. 1517-24, 1982