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anti-Mouse (Murine) Integrin beta 2 antibody for Immunoprecipitation

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • 2E6
  • AI256028
  • AI528527
  • CD18
  • Cd18
  • D12S370
  • ITGB2
  • LAD
  • LCAMB
  • LFA-1
  • Lfa1
  • LT-BETA-R
  • Ltar
  • LTbetaR
  • MAC-1
  • MF17
  • MFI7
  • TNF-R-III
  • Tnfbr
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFR3
  • TNFRrp
  • TNFRSF3
  • Tnfrsf3
Reactivity
Mouse (Murine)
469
208
111
39
39
21
20
14
3
3
2
2
2
2
2
2
2
1
1
Host
Rat
365
187
155
5
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
92
77
53
19
14
11
9
8
7
7
7
6
6
6
6
6
6
6
5
4
3
3
3
3
3
3
3
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
500
276
273
158
105
104
84
63
45
34
20
9
7
6
5
3
2
1
1
1
1
1
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Catalog No. ABIN319556
$ 176.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088800647 ABIN118149 FACS IHC (fro) IP Rat IgG2a Log in to see YTS 213-1 1
0.00088800647 ABIN457404 Func FACS IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see M18-2 10
0.00088800647 ABIN457430 Func FACS IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see M18-2 8
0.00088800647 ABIN280242 Func FACS IP IHC WB Rat IgG2a kappa Log in to see M18-2 1
0.00088800647 ABIN1176955 BR IHC (fro) FACS IP Rat IgG1, kappa Log in to see GAME-46 3
0.00088800647 ABIN439881 ELISA FACS ICC IHC IHC (fro) IHC (p) IP WB Biotin Rat IgG2a kappa Log in to see C71-16
0.00088800647 ABIN439882 FACS IHC (fro) IHC (p) IP IHC WB PE Rat IgG2a kappa Log in to see C71-16
0.00088800647 ABIN135144 FACS IP IHC WB Biotin Rat IgG2a Log in to see C71-16 1
0.00088800647 ABIN262325 FACS IHC (fro) IHC (p) IP IHC WB Rat IgG2a kappa Log in to see M18-2
0.00088800647 ABIN439880 FACS ICC IHC IHC (fro) IHC (p) IP WB FITC Rat IgG2a kappa Log in to see C71-16
0.00088800647 ABIN135142 FACS IP IHC WB Rat IgG2a Log in to see C71-16 1
0.00088800647 ABIN1584185 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.00088800647 ABIN2689033 BR IHC (f) IHC (fro) FACS IP WB Rat IgG2a, kappa Log in to see M18-2 7
0.00088800647 ABIN2689032 BR IHC (fro) FACS IP Rat IgG1, kappa Log in to see GAME 3
0.00088800647 ABIN316827 FACS IP Rat IgG2a Log in to see M18-2
0.00088800647 ABIN2490026 FACS IP WB Rat IgG2a Log in to see
0.00088800647 ABIN2490027 FACS IP WB Rat IgG2a Log in to see
0.00088800647 ABIN2490028 FACS IP WB Rat IgG2a Log in to see
0.00088800647 ABIN490178 Func FACS IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see M18-2
0.00088800647 ABIN4227582 BP FACS IP Neut Hamster IgG Log in to see 2-00E06 1

Top referenced anti-Integrin beta 2 antibody for Immunoprecipitation

Similar anti-Integrin beta 2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 6 Antibodies
ELISA 21 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 126 Antibodies
Functional Studies (Func) 17 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 59 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 75 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 2 Antibodies

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Mouse (Murine)
(469), (208), (111), (39), (39), (21), (20), (14), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(365), (187), (155), (5), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(92), (77), (53), (19), (14), (11), (9), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(500), (276), (273), (158), (105), (104), (84), (63), (45), (34), (20), (9), (7), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN319556) Images
Specificity This antibody reacts with Mouse CD18 (LFA-1 family b chain). YTS 213.1 does not block LFA-1 mediated T-cell activation in vitro. The antibody precipitates an antigen of 95-180 kDa.
Purification Affinity Chromatography on Protein G
Immunogen Mouse spleen cells. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 myeloma cell line.
Clone YTS213-1
Isotype IgG2a

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN319556) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18 antigen is a 90 kDa integral membrane glycoprotein, beta-2 chain, which can be non-covalently associated with each of the alpha chains of the beta-2 integrin family: alpha-L (gp160 - CD11a), alpha-M (gp155 - CD11b) or alpha-X (gp130 - CD11c). This molecule is involved in cell-cell interactions. Mouse CD18 (LFA-1 family b chain) is expressed on practically all types of leucocytes (platelets are negative).Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 16414
NCBI Accession NP_032430
UniProt P11835
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN319556) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/25 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN319556) Images back to top
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 Antibody (ABIN319556)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983