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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||2 Antibodies|
|Flow Cytometry (FACS)||27 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||2 Antibodies|
|Immunoprecipitation (IP)||16 Antibodies|
|Western Blotting (WB)||30 Antibodies|
|Antigen||Integrin beta 2 (ITGB2) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This Integrin beta 2 antibody is conjugated to Biotin Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|2 references available|
|Supplier||Log in to see|
Product Details anti-Integrin beta 2 AntibodyTarget Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN120418) Images
|Specificity||This Antibody is specific for the β subunit of LFA-1. This antibody also recognizes MAC-1 and p150, 95 as well as LFA-1, since they all contain the same β subunit. It inhibits homeotypic aggregation of PHA blasts and blocks the binding of rat lymphocytes to purified rat ICAM-1|
Target Details Integrin beta 2Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN120418) Images back to top
|Alternative Name||CD18 / ITGB2 (ITGB2 Antibody Abstract)|
|Background||LFA-1 (lymphocyte function associated molecule-1) is one of the leukocyte integrins. It is a heterodimer consisting of α and β subunits of 160-170 kDa and 95-100 kDa respectively. LFA-1 promotes non-antigen dependent adhesion of T-cells to a variety of lymphoid cells that bear its complementary receptor I-CAM-1 (1). It has a broad distribution and is found on most common lymphocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 Antibody (ABIN120418) Images back to top
Immunoprecipitation. Flow cytometry. Immunohistochemistry on cryostat sections. Functional studies: in vivo/in vitro.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 Antibody (ABIN120418) Images back to top
|Buffer||PBS, pH 7.2, with 0.09 % NaN3 and 0.5 % BSA.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Integrin beta 2 Antibody (ABIN120418)Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Yamazaki, Seko, Tamatani et al.: "Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules." in: The American journal of pathology, Vol. 143, Issue 2, pp. 410-8, 1993 (PubMed).
Tamatani, Kotani, Miyasaka: "Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies." in: European journal of immunology, Vol. 21, Issue 3, pp. 627-33, 1991 (PubMed).
ImagesProduct Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 Antibody (ABIN120418) back to top