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anti-Human IRAK1 antibody for Immunofluorescence

Recommended IRAK1 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
  • IRAK1
  • IRAK
  • pelle
  • AA408924
  • IRAK-1
  • IRAK1-S
  • Il1rak
  • Plpk
  • mPLK
  • RGD1563841
  • interleukin-1 receptor-associated kinase 1
  • IRAK1
  • irak1
  • Irak1
Epitope
C-Term
39
33
24
20
16
15
15
15
15
14
10
8
8
8
8
7
5
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
234
128
95
2
1
1
Host
Rabbit
223
42
2
Clonality
Polyclonal
Conjugate
This IRAK1 antibody is un-conjugated
12
11
10
6
6
6
4
4
4
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
188
121
66
52
49
42
33
16
15
9
8
3
1
1
Supplier
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Catalog No. ABIN500036
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.813965 ABIN614624 EIA IF IHC (p) WB Mouse IgG2a AA 530-693 Log in to see 3F7
11.813965 ABIN614625 EIA IF IHC (p) IP WB Mouse IgG1 AA 530-693 Log in to see 3A9
11.813965 ABIN4327500 ELISA ICC IF IP WB Rabbit Log in to see Polyclonal
11.813965 ABIN561509 IF IHC (p) IP ELISA WB Mouse IgG2a kappa AA 530-693, partial Log in to see 3F7
11.813965 ABIN561510 IF IHC (p) IP RNAi ELISA WB Mouse IgG1 kappa AA 530-693, partial Log in to see 3A9
11.813965 ABIN4327496 ICC IF IHC IHC (p) Rabbit Log in to see Polyclonal
1 ABIN401038 EIA IF IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 5
1 ABIN393719 IF WB Mouse IgG2a kappa AA 530-693 Log in to see 3F7 5
1 ABIN393660 IF WB Mouse IgG1 kappa AA 530-693 Log in to see 3A9 5
1 ABIN1450269 IF ELISA Rabbit IgG pThr387, Internal Region Log in to see Polyclonal 1
1 ABIN3185222 ELISA IF WB Rabbit IgG Ser602 Log in to see Polyclonal
1 ABIN272026 IF WB Rabbit Log in to see Polyclonal
1 ABIN2470982 IF IP RNAi IHC ELISA WB Mouse IgG1 kappa AA 530-693 Log in to see 3A9
1 ABIN2470981 IF IHC ELISA WB Mouse IgG2a kappa AA 530-693 Log in to see 3F7
1 ABIN574285 IF IHC (p) ELISA WB Mouse IgG2a, kappa AA 530-693 Log in to see 3F7
1 ABIN574286 IF IHC (p) IP RNAi ELISA WB Mouse IgG1, kappa AA 530-693 Log in to see 3A9
1 ABIN3183032 ELISA IF Rabbit IgG pThr387 Log in to see Polyclonal
1 ABIN121523 IF IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN4327492 ICC IF WB Rabbit Log in to see Polyclonal
1 ABIN2475129 IF IHC (p) WB Mouse IgG1 AA 530-693 Log in to see 3A9

Top referenced anti-IRAK1 antibody for Immunofluorescence

Similar anti-IRAK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 23 Antibodies 44 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 8 Antibodies
Immunofluorescence (IF) 10 Antibodies 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies 20 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Western Blotting (WB) 43 Antibodies
Flow Cytometry (FACS) 16 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) Antibodies
Epitope C-Term
(39), (33), (24), (20), (16), (15), (15), (15), (15), (14), (10), (8), (8), (8), (8), (7), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(234), (128), (95), (2), (1), (1)
Host Rabbit
(223), (42), (2)
Clonality
Polyclonal
Conjugate This IRAK1 antibody is un-conjugated
(12), (11), (10), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(188), (121), (66), (52), (49), (42), (33), (16), (15), (9), (8), (3), (1), (1)
Pubmed 5 references available
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Product Details anti-IRAK1 Antibody

Target Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN500036) Images
Specificity IRAK antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK.
Purification DEAE
Immunogen Human IRAK (C-Terminus) Peptide
Isotype IgG

Target Details IRAK1

Product Details anti-IRAK1 Antibody Application Details Handling References for anti-IRAK1 Antibody (ABIN500036) Images back to top
Antigen
Alternative Name IRAK1 (IRAK1 Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).Synonyms: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1
Gene ID 3654
UniProt P51617
Research Area Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-IRAK1 Antibody Target Details IRAK1 Handling References for anti-IRAK1 Antibody (ABIN500036) Images back to top
Application Notes ELISA. Western Blot: 1/1000. Immunocytochemistry. Immunoprecipitation: 2 to 4 μg per sample. An 80 kDa band should be detected in the lysate from non-activated cells. It has no crossresponse to IRAK2.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details References for anti-IRAK1 Antibody (ABIN500036) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-IRAK1 Antibody (ABIN500036)

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling Images back to top
Background publications

Yao, Li, Shi: "Optically active supramolecular complexes of water-soluble achiral polythiophenes and folic acid: spectroscopic studies and sensing applications." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 24, Issue 22, pp. 12829-35, 2008

Huang, Gao, Li, Cao: "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12829-32, 1998

Robinson, Shibuya, Mui, Zonin, Murphy, Sana, Hartley, Menon, Kastelein, Bazan, OGarra: "IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB." in: Immunity, Vol. 7, Issue 4, pp. 571-81, 1997

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996

Trofimova, Sprenkle, Green, Sturgill, Goebl, Harrington: "Developmental and tissue-specific expression of mouse pelle-like protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17609-12, 1996

Images

Product Details anti-IRAK1 Antibody Target Details IRAK1 Application Details Handling References for anti-IRAK1 Antibody (ABIN500036) back to top
Supplier Images
Immunofluorescence (IF) image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN500036) Immunocytochemistry of IRAK in HeLa cells with AP30438PU-N IRAK antibody at 10 μg/ml.
Western Blotting (WB) image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN500036) Western blot analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with AP...