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anti-Human IRAK4 antibody for Enzyme Immunoassay

Recommended IRAK4 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
  • zgc:55553
  • IRAK4
  • ipd1
  • irak-4
  • ny-ran-64
  • ny-ren-64
  • ren64
  • irak4
  • IPD1
  • IRAK-4
  • NY-REN-64
  • REN64
  • 8430405M07Rik
  • 9330209D03Rik
  • interleukin-1 receptor-associated kinase 4
  • Interleukin-1 receptor-associated kinase 4
  • IRAK4
  • irak4
  • LOC100227974
  • Irak4
Epitope
C-Term
40
31
16
15
15
15
14
6
6
6
5
5
5
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
190
55
27
13
5
4
4
4
2
2
2
2
1
Host
Rabbit
142
32
24
Clonality
Polyclonal
Conjugate
This IRAK4 antibody is un-conjugated
9
8
7
6
5
5
4
3
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
149
89
26
26
26
11
11
8
7
5
3
2
2
1
1
1
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Catalog No. ABIN401362
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN952943 EIA WB Rabbit Ig N-Term, AA 32-60 Log in to see Polyclonal 5
1 ABIN374511 EIA WB Goat N-Term Log in to see Polyclonal 1
1 ABIN500044 EIA IF WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN615818 EIA IHC (p) WB Goat N-Term Log in to see Polyclonal
1 ABIN1107881 EIA IHC (p) WB Mouse IgG1 Log in to see 2H9

Top referenced anti-IRAK4 antibody for Enzyme Immunoassay

Similar anti-IRAK4 Antibodies

Application / Reactivity Human
Dot Blot (DB) 1 Antibodies
ELISA 83 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 25 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 142 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
Epitope C-Term
(40), (31), (16), (15), (15), (15), (14), (6), (6), (6), (5), (5), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(190), (55), (27), (13), (5), (4), (4), (4), (2), (2), (2), (2), (1)
Host Rabbit
(142), (32), (24)
Clonality
Polyclonal
Conjugate This IRAK4 antibody is un-conjugated
(9), (8), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(149), (89), (26), (26), (26), (11), (11), (8), (7), (5), (3), (2), (2), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-IRAK4 Antibody

Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN401362) Images
Specificity This antibody is directed against IRAK-4 protein.
Purification Affinity chromatography
Immunogen Synthetic peptide corresponding to a region near the carboxy terminus of human IRAK-4 protein
Isotype IgG

Target Details IRAK4

Product Details anti-IRAK4 Antibody Application Details Handling References for anti-IRAK4 Antibody (ABIN401362) Images back to top
Antigen
Alternative Name IRAK4 (IRAK4 Antibody Abstract)
Background IRAK-4 (Interleukin-1 receptor-associated kinase 4). Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR (1-3). A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4 (4,5). Overexpression of IRAK-4 activates NF-kappaB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges (5,6). Members of the IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.Synonyms: IRAK-4, Interleukin-1 receptor-associated kinase-like, NY-REN-64
Gene ID 51135
NCBI Accession NP_001107654
UniProt Q9NWZ3
Research Area Immunology, Innate Immunity, Atherosclerosis, Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-IRAK4 Antibody Target Details IRAK4 Handling References for anti-IRAK4 Antibody (ABIN401362) Images back to top
Application Notes ELISA: 1/5,000 - 1/20,000. Western Blot: 1/250 - 1/1,000. Immunohistochemistry: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details References for anti-IRAK4 Antibody (ABIN401362) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-IRAK4 Antibody (ABIN401362)

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling Images back to top
Background publications

Picard, Puel, Bonnet, Ku, Bustamante, Yang, Soudais, Dupuis, Feinberg, Fieschi, Elbim, Hitchcock, Lammas, Davies, Al-Ghonaium, Al-Rayes, Al-Jumaah, Al-Hajjar, Al-Mohsen, Frayha, Rucker, Hawn, Aderem et al.: "Pyogenic bacterial infections in humans with IRAK-4 deficiency. ..." in: Science (New York, N.Y.), Vol. 299, Issue 5615, pp. 2076-9, 2003

Li, Strelow, Fontana, Wesche: "IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 8, pp. 5567-72, 2002

Suzuki, Suzuki, Duncan, Millar, Wada, Mirtsos, Takada, Wakeham, Itie, Li, Penninger, Wesche, Ohashi, Mak, Yeh: "Severe impairment of interleukin-1 and Toll-like receptor signalling in mice lacking IRAK-4." in: Nature, Vol. 416, Issue 6882, pp. 750-6, 2002

Wesche, Gao, Li, Kirschning, Stark, Cao: "IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family." in: The Journal of biological chemistry, Vol. 274, Issue 27, pp. 19403-10, 1999

Muzio, Ni, Feng, Dixit: "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1612-5, 1997

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996

Branlant, Krol, Ebel: "Characterization of the Escherichia coli 23S Ribosomal RNA region associated with ribosomal protein L1. Evidence for homologies with the 5'-end region of the L11 operon." in: Nucleic acids research, Vol. 8, Issue 23, pp. 5567-77, 1981

Images

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN401362) back to top
Supplier Images
 image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (C-Term) (ABIN401362) anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (C-Term) antibody
 image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (C-Term) (ABIN401362) anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (C-Term) antibody (Image 2)