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anti-Human IRAK4 antibody for Flow Cytometry

Recommended IRAK4 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
  • 8430405M07Rik
  • 9330209D03Rik
  • IPD1
  • ipd1
  • irak-4
  • IRAK-4
  • irak4
  • IRAK4
  • ny-ran-64
  • NY-REN-64
  • ny-ren-64
  • ren64
  • REN64
  • zgc:55553
Reactivity
Human, Mouse (Murine), Monkey
173
44
18
5
2
1
1
1
1
1
1
1
Host
Mouse
132
27
25
Clonality
Monoclonal
Conjugate
This IRAK4 antibody is un-conjugated
9
8
7
6
5
5
4
3
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
139
82
25
22
18
10
9
7
4
3
3
2
2
2
1
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Catalog No. ABIN969218
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30704167 ABIN1845968 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.0010664745 ABIN1959887 FACS IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 2H9
0.0010664745 ABIN2290955 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B423

Top referenced anti-IRAK4 antibody for Flow Cytometry

Similar anti-IRAK4 Antibodies

Application / Reactivity Mouse (Murine) Monkey Human
ELISA 16 Antibodies 3 Antibodies 77 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 2 Antibodies 7 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies 3 Antibodies
Western Blotting (WB) 32 Antibodies 5 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
Reactivity Human, Mouse (Murine), Monkey
(173), (44), (18), (5), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(132), (27), (25)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IRAK4 antibody is un-conjugated
(9), (8), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(139), (82), (25), (22), (18), (10), (9), (7), (4), (3), (3), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-IRAK4 Antibody

Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN969218) Images
Purification Ascites
Immunogen Purified recombinant fragment of human IRAK4 expressed in E. coli.
Clone 2H9
Isotype IgG1

Target Details IRAK4

Product Details anti-IRAK4 Antibody Application Details Handling References for anti-IRAK4 Antibody (ABIN969218) Images back to top
Antigen
Alternative Name IRAK4 (IRAK4 Antibody Abstract)
Background This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 52 kDa
Gene ID 51135
HGNC 51135
Research Area Immunology, Innate Immunity, Atherosclerosis, Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling

Application Details

Product Details anti-IRAK4 Antibody Target Details IRAK4 Handling References for anti-IRAK4 Antibody (ABIN969218) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details References for anti-IRAK4 Antibody (ABIN969218) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-IRAK4 Antibody (ABIN969218)

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling Images back to top
Product cited in:

Dunne, Carpenter, Brikos, Gray, Strelow, Wesche, Morrice, ONeill: "IRAK1 and IRAK4 promote phosphorylation, ubiquitination, and degradation of MyD88 adaptor-like (Mal)." in: The Journal of biological chemistry, Vol. 285, Issue 24, pp. 18276-82, 2010

Pathak, De Souza, Salte, Wiker, Asjö: "HIV induces both a down-regulation of IRAK-4 that impairs TLR signalling and an up-regulation of the antibiotic peptide dermcidin in monocytic cells." in: Scandinavian journal of immunology, Vol. 70, Issue 3, pp. 264-76, 2009

Images

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN969218) back to top
Supplier Images
ELISA image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN969218) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN969218) Figure 2: Flow cytometric analysis of Hela cells using IRAK4 mouse mAb (blue) and neg...
Immunohistochemistry (IHC) image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN969218) Figure 3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues...
Immunohistochemistry (IHC) image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN969218) Figure 4: Immunohistochemical analysis of paraffin-embedded human kidney cancer tissu...
Western Blotting (WB) image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN969218) Figure 5: Western blot analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K5...
Western Blotting (WB) image for anti-IRAK4 antibody (Interleukin-1 Receptor-Associated Kinase 4) (ABIN969218) Figure 6: Western blot analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) reco...