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anti-Human IRAK4 antibody for Intracellular Staining

Recommended IRAK4 Antibody (supplied by: Log in to see )

Antigen
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
  • 8430405M07Rik
  • 9330209D03Rik
  • IPD1
  • ipd1
  • irak-4
  • IRAK-4
  • irak4
  • IRAK4
  • ny-ran-64
  • NY-REN-64
  • ny-ren-64
  • ren64
  • REN64
  • zgc:55553
Reactivity
Human
173
45
18
6
2
1
1
1
1
1
1
1
Host
Mouse
132
27
25
Clonality
Monoclonal
Conjugate
This IRAK4 antibody is conjugated to PE
9
8
7
5
5
5
4
3
2
2
2
2
2
2
2
2
Application
Intracellular Staining (ICS)
140
83
25
22
19
10
9
7
4
4
3
2
2
1
1
Supplier
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Catalog No. ABIN1176904
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087247934 ABIN1176905 ICS Alexa Fluor 647 Mouse IgG1, kappa Log in to see L29-525 4

Top referenced anti-IRAK4 antibody for Intracellular Staining

Similar anti-IRAK4 Antibodies

Application / Reactivity Human
ELISA 77 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 131 Antibodies

General

Antigen Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Antibodies
Reactivity Human
(173), (45), (18), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(132), (27), (25)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IRAK4 antibody is conjugated to PE
(9), (8), (7), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Intracellular Staining (ICS)
(140), (83), (25), (22), (19), (10), (9), (7), (4), (4), (3), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-IRAK4 Antibody

Target Details IRAK4 Application Details Handling References for anti-IRAK4 Antibody (ABIN1176904) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human IRAK4 Recombinant Protein
Clone L29-525
Isotype IgG1, kappa

Target Details IRAK4

Product Details anti-IRAK4 Antibody Application Details Handling References for anti-IRAK4 Antibody (ABIN1176904) Images back to top
Antigen
Alternative Name IRAK4 (IRAK4 Antibody Abstract)
Background Interleukin 1 (IL-1) is a proinflammatory cytokine that mediates the host inflammatory response. IL-1 exerts its biological effects through its type I receptor (IL-1RI), which is found on most cell types. When IL-1 binds to the IL-1RI, NF-kappaB (transcription factor) is activated and translocates to the nucleus where it induces gene expression. IL-1-receptor-associated kinase (IRAK) has been reported to co-immunoprecipitate with IL-1RI. The IRAK family is made up of four identified Pelle homologs that regulate the activation of NF-kappaB and mitogen-activated protein (MAP) kinase pathways. IRAK4 is a unique member of the IRAK family because it is the closest mammalian homolog to Pelle. Also, when overexpressed, its kinase activity allows it to activate both the NF-kappaB and mitogen-activated protein (MAP) kinase pathways, and block the IL-1-induced activation and modification of IRAK1. IRAK4 is also able to phosphorylate IRAK1, which suggests that IRAK4 affects the early signal transduction of Toll/IL-1 receptors before the involvement of IRAK1. The L29-525 monoclonal antibody recognizes human IRAK4, regardless of phosphorylation status. The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.
Synonyms: IRAK-4, REN64, Q69FE3
Research Area Inflammation
Pathways NF-kappaB Signaling, TLR Signaling

Application Details

Product Details anti-IRAK4 Antibody Target Details IRAK4 Handling References for anti-IRAK4 Antibody (ABIN1176904) Images back to top
Application Notes This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells and cell lines (using BD™ Phosflow Lyse/Fix Buffer or BD Cytofix™ Fixation Buffer), but not human whole blood. BD™ Phosflow Perm Buffer II or Perm Buffer III may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details References for anti-IRAK4 Antibody (ABIN1176904) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Confirmation of the specificity of mAb L29-525 by western blot analysis (left panel). Lysates of human PBMC were probed with unconjugated antibody at a concentration of 0.1 μg/ml. IRAK4 is identified as a band of about 52 kDa. Western blot validation of IRAK4 by RNAi (right panel). Lysates from HeLa S3 cells (lane 1) and IRAK4 RNAi-transfected HeLa S3 cells (lane 2) were probed with unconjugated mAb L29-525 at 0.015 μg/ml. Down-regulation of IRAK4 expression is evident in the RNAi-transfected cells.

References for anti-IRAK4 Antibody (ABIN1176904)

Product Details anti-IRAK4 Antibody Target Details IRAK4 Application Details Handling Images back to top
Product cited in:

Suzuki, Saito: "IRAK-4--a shared NF-kappaB activator in innate and acquired immunity." in: Trends in immunology, Vol. 27, Issue 12, pp. 566-72, 2006

Hatao, Muroi, Hiki, Ogawa, Mimura, Kaminishi, Tanamoto: "Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein." in: Journal of leukocyte biology, Vol. 76, Issue 4, pp. 904-8, 2004

Lye, Mirtsos, Suzuki, Suzuki, Yeh: "The role of interleukin 1 receptor-associated kinase-4 (IRAK-4) kinase activity in IRAK-4-mediated signaling." in: The Journal of biological chemistry, Vol. 279, Issue 39, pp. 40653-8, 2004

Li, Strelow, Fontana, Wesche: "IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 8, pp. 5567-72, 2002