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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||142 Antibodies||67 Antibodies||54 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies||3 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||17 Antibodies||16 Antibodies||12 Antibodies|
|Immunohistochemistry (IHC)||48 Antibodies||22 Antibodies||13 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP3K7 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
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Product Details anti-MAP3K7 AntibodyTarget Details MAP3K7 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Tak1 protein. (region surrounding Asp181)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details MAP3K7Product Details anti-MAP3K7 Antibody Application Details Handling Images back to top
|Alternative Name||TAK1 / MAP3K7 (MAP3K7 Antibody Abstract)|
|Background||Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a 74 kDa protein with intrinsic kinase activity towards serine/threonine residues and that is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFß stimulation.Synonyms: Mitogen-activated protein kinase kinase kinase 7, TAK-1, TAK1, TGF-beta-activated kinase 1, Transforming growth factor-beta-activated kinase 1|
|Molecular Weight||approx. 70 to 82 kDa|
|Pathways||NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAP3K7 Antibody Target Details MAP3K7 Handling Images back to top
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K7 Antibody Target Details MAP3K7 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-MAP3K7 Antibody Target Details MAP3K7 Application Details Handling back to top