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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Competition ELISA|
|Detection Range||1.4-1000 pg/mL|
|Minimum Detection Limit||1.4 pg/mL|
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Product Details MAP3K7 ELISA KitTarget details Application Details Handling Images
|Purpose||The kit is a competitive enzyme immunoassay for the in vitro quantitative measurement of TGF-1 alpha in human serum, plasma, andcell culture media|
|Sample Type||Cell Culture Supernatant, Plasma, Serum|
|Specificity||This assay has high sensitivity and excellent specificity for detection of TGF-1 alpha|
|Cross-Reactivity (Details)||No significant cross-activity was observed between this target and other analogues|
Microplate: 96 breakable wells (12strips x 8wells) coated with anti-human TGF-1 alpha.
|Material not included||
Target detailsProduct Details MAP3K7 ELISA Kit Application Details Handling Images back to top
|Alternative Name||TGF-1 alpha (MAP3K7 ELISA Kit Abstract)|
|Pathways||NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Toll-Like Receptors Cascades|
Application DetailsProduct Details MAP3K7 ELISA Kit Target details Handling Images back to top
|Sample Volume||100 μL|
|Assay Time||4.5 h|
|Protocol||The Human TGF-1 alpha ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TGF-1 alpha in human serum and plasma. An antibody specific for human TGF-1 alpha was coated on a 96-well plate. Standards and samples are added to the wells and any TGF-1 alpha present binds to the immobilized antibody. The wells are washed and biotinylated anti-human TGF-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TGF-1 alpha present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm|
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles. Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 x g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.|
|Assay Precision||intra CV<10%, inter CV <15%|
|Restrictions||For Research Use only|
HandlingProduct Details MAP3K7 ELISA Kit Target details Application Details Images back to top
|Storage||4 °C,-20 °C|
|Storage Comment||4°C/-20°C,May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.|