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Human MYD88 ELISA Kit

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Antigen
Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA Kits
  • CG2078
  • Dmel\\CG2078
  • DmMyd88
  • DmMyD88
  • DMMYD88
  • dMyD88
  • dMyd88
  • EP(2)2535
  • GB12344
  • irak-2
  • Kra
  • kra
  • LD20892
  • MGC84928
  • myd88
  • MYD88
  • MyD88
  • myd88d
  • MYD88D
  • Myd88F
  • NV10640
  • XMyD88
  • zgc:103541
Epitope
AA 1-296
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
8
4
4
2
1
1
Methode Type
Sandwich ELISA
Detection Range
312-20000 pg/mL
Minimum Detection Limit
312 pg/mL
Application
ELISA
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Catalog No. ABIN1672854
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666156 ABIN414798 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.32666156 ABIN1029341 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32666156 ABIN2708338 Sandwich ELISA Cell Culture Supernatant 312-20000 pg/mL 312 pg/mL Log in to see
0.00121214 ABIN455936 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.00121214 ABIN2042614 Sandwich ELISA 2-20000 pg/mL 2 pg/mL Log in to see
0.00121214 ABIN2685042 Sandwich ELISA Cell Culture Supernatant 312-20000 pg/mL 312 pg/mL Log in to see
0.00121214 ABIN2092823 Sandwich ELISA Log in to see
0.00121214 ABIN2949667 0.156-10 ng/mL 0.156 ng/mL Log in to see

Top referenced MYD88 ELISA Kit for ELISA

Similar MYD88 ELISA Kits

Application / Reactivity Human
ELISA 9 ELISA Kits

General

Antigen Myeloid Differentiation Primary Response Gene (88) (MYD88) ELISA Kits
Epitope AA 1-296
Reactivity Human
Kits with alternative reactivity to:
(8), (4), (4), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 312-20000 pg/mL
Minimum Detection Limit 312 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details MYD88 ELISA Kit

Target details Application Details Handling References for MYD88 Kit (ABIN1672854) Images
Purpose For quantitative detection of human MyD88 in cell culture supernates.
Brand PicoKine™
Sample Type Cell Culture Supernatant
Detection Method Colorimetric
Specificity Natural and recombinant human MyD88
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human MyD88 antibod - 1
  • Lyophilized recombinant human MyD88 standar - 20ng/tubex2
  • Biotinylated anti- human MyD88 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: M1-P296

Target details

Product Details MYD88 ELISA Kit Application Details Handling References for MYD88 Kit (ABIN1672854) Images back to top
Antigen
Alternative Name MYD88 (MYD88 ELISA Kit Abstract)
Background Myeloid differentiation primary response gene(88)(MYD88) is a protein that, in humans, is encoded by the MYD88 gene. By interspecific backcross mapping, the mouse MyD88 gene was localized to chromosome 9, the human homolog was mapped to 3p22-p21.3 The human ortholog MYD88 seems to function similarly to mice, however, available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses.
Gene ID 4615
UniProt Q99836
Pathways NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human MyD88 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MyD88 has been precoated onto 96-well plates. Standards(E.coli, M1-P296) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MyD88 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MyD88 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MyD88 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MyD88 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 2.1, Standard deviation: 0.13, CV(%): 6.2
  • Sample 2: n=16, Mean(ng/ml): 7, Standard deviation: 0.371, CV(%): 5.3
  • Sample 3: n=16, Mean(ng/ml): 13.7, Standard deviation: 0.7, CV(%): 5.1,
  • Sample 1: n=24, Mean(ng/ml): 2.7, Standard deviation: 0.189, CV(%): 7
  • Sample 2: n=24, Mean(ng/ml): 8.6, Standard deviation: 0.559, CV(%): 6.5
  • Sample 3: n=24, Mean(ng/ml): 15.2, Standard deviation: 0.882, CV(%): 5.8
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for MYD88 Kit (ABIN1672854)

Product Details MYD88 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Bonnert, Garka, Parnet, Sonoda, Testa, Sims: "The cloning and characterization of human MyD88: a member of an IL-1 receptor related family." in: FEBS letters, Vol. 402, Issue 1, pp. 81-4, 1997

Images

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Supplier Images
ELISA image for MYD88 ELISA Kit (Myeloid Differentiation Primary Response Gene (88)) (ABIN1672854) Human MyD88 PicoKine ELISA Kit standard curve