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anti-Human NFKB1 antibody for Dot Blot

Recommended NFKB1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
  • BICDR-1
  • bicdr1
  • ccdc64
  • EBP-1
  • ebp-1
  • KBF1
  • kbf1
  • NF-kappa-B
  • nf-kappa-b
  • nf-kappaB
  • NF-kappaB
  • NF-kappaB1
  • NF-kB
  • NF-KB1
  • NF-kB1
  • NFkappaB
  • NFKB-p50
  • nfkb-p50
  • nfkb-p105
  • NFKB-p105
  • p50
  • p50/p105
  • p105
Epitope
pSer536
44
43
42
39
38
36
28
15
13
13
13
11
9
8
7
7
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
524
157
137
17
17
14
12
1
1
Host
Rabbit
476
72
4
2
Clonality
Polyclonal
Conjugate
This NFKB1 antibody is un-conjugated
19
16
14
7
7
6
6
6
6
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB)
423
218
173
111
105
68
44
29
16
9
8
7
4
4
4
1
1
1
Supplier
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Catalog No. ABIN650886
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008934913 ABIN2324521 DB ELISA Rabbit IgG pSer536 Log in to see Polyclonal
0.0008934913 ABIN2324544 DB ELISA APC Rabbit IgG pSer536 Log in to see Polyclonal
0.0008934913 ABIN2324550 DB ELISA FITC Rabbit IgG pSer536 Log in to see Polyclonal
0.0008934913 ABIN2324553 DB ELISA HRP Rabbit IgG pSer536 Log in to see Polyclonal
0.0008934913 ABIN2324556 DB ELISA PE Rabbit IgG pSer536 Log in to see Polyclonal
0.0008934913 ABIN2324541 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pSer536 Log in to see Polyclonal
0.0008934913 ABIN2324547 DB ELISA Biotin Rabbit IgG pSer536 Log in to see Polyclonal
0.0008934913 ABIN2794335 DB Rabbit Ig Fraction pSer536 Log in to see Polyclonal

Top referenced anti-NFKB1 antibody for Dot Blot

Similar anti-NFKB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 9 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 166 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Gel Shift (GS) 7 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 57 Antibodies
Immunohistochemistry (IHC) 217 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 108 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
Epitope pSer536
(44), (43), (42), (39), (38), (36), (28), (15), (13), (13), (13), (11), (9), (8), (7), (7), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(524), (157), (137), (17), (17), (14), (12), (1), (1)
Host Rabbit
(476), (72), (4), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This NFKB1 antibody is un-conjugated
(19), (16), (14), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB)
(423), (218), (173), (111), (105), (68), (44), (29), (16), (9), (8), (7), (4), (4), (4), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-NFKB1 Antibody

Target Details NFKB1 Application Details Handling References for anti-NFKB1 Antibody (ABIN650886) Images
Specificity This Phospho-NF kappa B-S536 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding S536 of human NF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NF kappa B Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NF kappa B.
Clone RB22552
Isotype Ig

Target Details NFKB1

Product Details anti-NFKB1 Antibody Application Details Handling References for anti-NFKB1 Antibody (ABIN650886) Images back to top
Antigen
Alternative Name NF kappa B (NFKB1 Antibody Abstract)
Background NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Synonyms: Transcription factor p65,RELA,NFKB3
Molecular Weight 60219 DA
Gene ID 5970
NCBI Accession NP_068810, NP_001138610
UniProt Q04206
Research Area Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-NFKB1 Antibody Target Details NFKB1 Handling References for anti-NFKB1 Antibody (ABIN650886) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details References for anti-NFKB1 Antibody (ABIN650886) Images back to top
Format Liquid
Concentration 0.31 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-NFKB1 Antibody (ABIN650886)

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Handling Images back to top
Background publications

Pan, Li, Huang, Papadimos, Pan, Chen: "Synergistic activation of NF-{kappa}B by bacterial chemoattractant and TNF{alpha} is mediated by p38 MAPK-dependent RelA acetylation." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34348-54, 2010

Tago, Funakoshi-Tago, Sakinawa, Mizuno, Itoh: "KappaB-Ras is a nuclear-cytoplasmic small GTPase that inhibits NF-kappaB activation through the suppression of transcriptional activation of p65/RelA." in: The Journal of biological chemistry, Vol. 285, Issue 40, pp. 30622-33, 2010

Images

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Handling References for anti-NFKB1 Antibody (ABIN650886) back to top
Supplier Images
Dot Blot (DB) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer536) (ABIN650886) Dot blot analysis of anti-Phospho-NF kappa B-S536 Phospho-specific Pab (ABIN650886) o...