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anti-Human NFKB1 antibody for Electrophoretic Mobility-Shift Assay

Recommended NFKB1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
  • BICDR-1
  • bicdr1
  • ccdc64
  • EBP-1
  • ebp-1
  • KBF1
  • kbf1
  • NF-kappa-B
  • nf-kappa-b
  • nf-kappaB
  • NF-kappaB
  • NF-kappaB1
  • NF-kB
  • NF-KB1
  • NF-kB1
  • NFkappaB
  • NFKB-p50
  • nfkb-p50
  • nfkb-p105
  • NFKB-p105
  • p50
  • p50/p105
  • p105
Epitope
N-Term
43
43
42
39
38
36
28
15
13
13
13
11
10
8
7
7
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
524
157
137
17
17
14
12
1
1
Host
Rabbit
476
72
4
2
Clonality
Polyclonal
Conjugate
This NFKB1 antibody is un-conjugated
19
16
14
7
7
6
6
6
6
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
422
218
173
111
105
68
43
29
16
9
8
7
4
4
4
1
1
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Catalog No. ABIN116520
$ 500.50
Plus shipping costs $45.00

Similar anti-NFKB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 9 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 166 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Gel Shift (GS) 7 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 57 Antibodies
Immunohistochemistry (IHC) 217 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 108 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
Epitope N-Term
(43), (43), (42), (39), (38), (36), (28), (15), (13), (13), (13), (11), (10), (8), (7), (7), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(524), (157), (137), (17), (17), (14), (12), (1), (1)
Host Rabbit
(476), (72), (4), (2)
Clonality
Polyclonal
Conjugate This NFKB1 antibody is un-conjugated
(19), (16), (14), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(422), (218), (173), (111), (105), (68), (43), (29), (16), (9), (8), (7), (4), (4), (4), (1), (1)
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Product Details anti-NFKB1 Antibody

Target Details NFKB1 Application Details Handling Images
Specificity Anti-NFkB p50 may react non-specifically with other proteins. Control peptide (#R1008PEP) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1). In immunoblot no reaction was observed against the analogous Mmouse protein.
Purification This product was prepared from monospecific antiserum by delipidation and defibrination.
Immunogen Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH)

Target Details NFKB1

Product Details anti-NFKB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NF-kB p105 / p50 (NFKB1 Antibody Abstract)
Background NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52(NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-alpha. IkB-alpha binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.Synonyms: EBP-1, EBP1, KBF1, NF-kappa-B p50, NFKB1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Gene ID 4790
NCBI Accession NP_001158884
UniProt P19838
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-NFKB1 Antibody Target Details NFKB1 Handling Images back to top
Application Notes Suitable for use in Immunoprecipitation, Immunoblotting (1/500-1/2,000), ELISA(1/5,000-1/25,000) and Supershift assays. This product was assayed by Immunoblot and found to be reactive against Human NFkBp50 (NFKB1) at a dilution of 1/1000 followed by reaction with Peroxidase conjugatedanti-Rabbit IgG [H&L] R1364HRP. Anti-Human NFkB p50 (NFKB1) is suitable for the detectionby Immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105. This product was also tested in a Gel Supershift assay and found to be reactive againstp50: p50 homodimers and p: 50: p65 heterodimers using 0.5 to 1.0 μL per assay. Gel (Super) Shift Information: In general, NFkB gel shift assays are assembled in 20 μL reactions containing 0.28 pmolesNFkB oligo in 10 mM Tris ( pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10 % glycerol. Some procedures specify the addition of 0.05 % NP-40. When using purified protein,250-300 ng should be sufficient to produce a gel shifted complex, while 10 ?g HeLa nuclear
Restrictions For Research Use only

Handling

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Images back to top
Format Liquid
Concentration 80.0 mg/mL (by Refractometry)
Precaution of Use This product contains sodium azide : a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (N-Term) (ABIN116520) Figure. Immunoblot of HeLa cell extract. All incubations except color development