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anti-Human NFKB1 antibody for Flow Cytometry

Recommended NFKB1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
  • BICDR-1
  • bicdr1
  • ccdc64
  • EBP-1
  • ebp-1
  • KBF1
  • kbf1
  • NF-kappa-B
  • nf-kappa-b
  • nf-kappaB
  • NF-kappaB
  • NF-kappaB1
  • NF-kB
  • NF-KB1
  • NF-kB1
  • NFkappaB
  • NFKB-p50
  • nfkb-p50
  • nfkb-p105
  • NFKB-p105
  • p50
  • p50/p105
  • p105
Reactivity
Human
499
154
135
17
17
14
12
1
1
Host
Mouse
472
51
4
2
Clonality
Monoclonal
Conjugate
This NFKB1 antibody is un-conjugated
18
16
13
6
6
6
6
6
6
6
6
6
5
5
5
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
397
210
170
101
86
68
42
24
13
9
9
7
4
4
4
1
1
1
Supplier
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Catalog No. ABIN969313
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN969312 FACS IHC ELISA WB Mouse IgG2a Log in to see 5D10
0.3068567 ABIN1845898 FACS IHC ELISA WB Mouse IgG2a Log in to see
0.3068567 ABIN1845899 ICC FACS IF IHC ELISA WB Mouse IgG2a Log in to see
0.0010666476 ABIN189591 ICC FACS IF IHC (p) IHC WB Rabbit IgG Log in to see E381
0.0010666476 ABIN1499721 FACS IHC ELISA WB Mouse IgG2a Log in to see 5D10
0.0010666476 ABIN1499720 ICC FACS IF IHC ELISA WB Mouse IgG2a Log in to see 5D10D11
0.0010666476 ABIN966667 FACS IHC ELISA WB Mouse IgG2a Log in to see 5D10 2
0.0010666476 ABIN966668 FACS IF IHC ELISA WB Mouse IgG2a Log in to see 5D10D11 2
0.0010666476 ABIN865404 FACS IHC ELISA WB Mouse IgG1 Log in to see 5D10
0.0010666476 ABIN865396 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D10D11
0.0010666476 ABIN2586134 FACS Mouse IgG1, kappa Log in to see 1298CT792-105-117-133
0.0010666476 ABIN2325547 FACS IHC ELISA WB Mouse IgG2a Log in to see 13B568
0.0010666476 ABIN2325552 FACS IHC ELISA WB Mouse IgG2a Log in to see 13B567
0.0010666476 ABIN3032043 FACS IHC ELISA WB Mouse IgG1, kappa Log in to see 1298CT792-105-117-133

Top referenced anti-NFKB1 antibody for Flow Cytometry

Similar anti-NFKB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 9 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 164 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Gel Shift (GS) 7 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 103 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 57 Antibodies
Immunohistochemistry (IHC) 210 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 83 Antibodies
Immunoprecipitation (IP) 42 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
Reactivity Human
(499), (154), (135), (17), (17), (14), (12), (1), (1)
Host Mouse
(472), (51), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKB1 antibody is un-conjugated
(18), (16), (13), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(397), (210), (170), (101), (86), (68), (42), (24), (13), (9), (9), (7), (4), (4), (4), (1), (1), (1)
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Product Details anti-NFKB1 Antibody

Target Details NFKB1 Application Details Handling Images
Purification Ascites
Immunogen Purified recombinant fragment of human NFKB1 expressed in E. coli.
Clone 5D10D11
Isotype IgG2a

Target Details NFKB1

Product Details anti-NFKB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NFKB1 (NFKB1 Antibody Abstract)
Background This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 50 kDa/105 kDa
Gene ID 4790
HGNC 4790
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-NFKB1 Antibody Target Details NFKB1 Handling Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

Images

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Handling back to top
Supplier Images
ELISA image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN969313) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN969313) Figure 2: Flow cytometric analysis of MCF-7 cells using NFKB1 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN969313) Figure 3: Immunofluorescence analysis of U251 cells using NFKB1 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN969313) Figure 4: Immunohistochemical analysis of paraffin-embedded human intima cancer tissu...
Western Blotting (WB) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN969313) Figure 5: Western blot analysis using NFKB1 mouse mAb against K562 (1), Jurkat (2), A...