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anti-Human NFKB1 antibody for Intracellular Staining

Recommended NFKB1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
  • BICDR-1
  • bicdr1
  • ccdc64
  • EBP-1
  • ebp-1
  • KBF1
  • kbf1
  • NF-kappa-B
  • nf-kappa-b
  • nf-kappaB
  • NF-kappaB
  • NF-kappaB1
  • NF-kB
  • NF-KB1
  • NF-kB1
  • NFkappaB
  • NFKB-p50
  • nfkb-p50
  • nfkb-p105
  • NFKB-p105
  • p50
  • p50/p105
  • p105
Epitope
pSer529
44
43
42
39
38
36
28
15
13
13
13
11
10
8
7
7
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
524
157
137
17
17
14
12
1
1
Host
Mouse
477
71
4
2
Clonality
Monoclonal
Conjugate
This NFKB1 antibody is un-conjugated
19
16
14
7
7
6
6
6
6
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS), Western Blotting (WB)
422
218
173
111
105
68
44
29
16
9
9
7
4
4
4
1
1
Supplier
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Catalog No. ABIN967610
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Top referenced anti-NFKB1 antibody for Intracellular Staining

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General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies
Epitope pSer529
(44), (43), (42), (39), (38), (36), (28), (15), (13), (13), (13), (11), (10), (8), (7), (7), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(524), (157), (137), (17), (17), (14), (12), (1), (1)
Host Mouse
(477), (71), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKB1 antibody is un-conjugated
(19), (16), (14), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS), Western Blotting (WB)
(422), (218), (173), (111), (105), (68), (44), (29), (16), (9), (9), (7), (4), (4), (4), (1), (1)
Pubmed 3 references available
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Product Details anti-NFKB1 Antibody

Target Details NFKB1 Application Details Handling References for anti-NFKB1 Antibody (ABIN967610) Images
Brand BD Pharmingen™
Characteristics 1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human NF-kappaB p65 Peptide
Clone K10-895-12-50
Isotype IgG2b, kappa

Target Details NFKB1

Product Details anti-NFKB1 Antibody Application Details Handling References for anti-NFKB1 Antibody (ABIN967610) Images back to top
Antigen
Alternative Name NF-kappa B (NFKB1 Antibody Abstract)
Background Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF-kB p65 subunit.
Molecular Weight 65 kDa
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-NFKB1 Antibody Target Details NFKB1 Handling References for anti-NFKB1 Antibody (ABIN967610) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details References for anti-NFKB1 Antibody (ABIN967610) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-NFKB1 Antibody (ABIN967610)

Product Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Handling Images back to top
Product cited in:

Natoli, Saccani, Bosisio, Marazzi: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." in: Nature immunology, Vol. 6, Issue 5, pp. 439-45, 2005

Siebenlist, Brown, Claudio: "Control of lymphocyte development by nuclear factor-kappaB." in: Nature reviews. Immunology, Vol. 5, Issue 6, pp. 435-45, 2005

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005

Images

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Supplier Images
Western Blotting (WB) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer529) (ABIN967610) Western blot analysis of NF-kappaB p65 (pS529) in transformed human epithelioid carci...
Western Blotting (WB) image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer529) (ABIN967610) Western blot analysis of NF-kappaB p65 (pS529) in human peripheral blood mononuclear ...
 image for anti-NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (pSer529) (ABIN967610) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer529) antibody (Image 3)