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|Antigen||Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This NFKB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-NFKB1 AntibodyTarget Details NFKB1 Application Details Handling Images
|Specificity||NFkappaB-p105/p50 antibody detects endogenous levels of NFkappaB-p105/p50 protein.(region surrounding Val331)|
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details NFKB1Product Details anti-NFKB1 Antibody Application Details Handling Images back to top
|Alternative Name||NF-kB p105 / p50 (NFKB1 Antibody Abstract)|
|Background||Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFkappaB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin kappa light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkappaB is activated and NFkappaB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.Synonyms: EBP-1, EBP1, KBF1, NF-kappa-B p50, NFKB1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1|
|Pathways||p53 Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-NFKB1 Antibody Target Details NFKB1 Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Handling back to top