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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||11 Antibodies||9 Antibodies||6 Antibodies|
|Western Blotting (WB)||455 Antibodies||187 Antibodies||146 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Immunoprecipitation (IP)||53 Antibodies||11 Antibodies||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||127 Antibodies||42 Antibodies||36 Antibodies|
|Immunohistochemistry (IHC)||226 Antibodies||103 Antibodies|
|Antigen||Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This NFKB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-NFKB1 AntibodyTarget Details NFKB1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of NFκB-p105/p50 protein. (region surrounding Asp926)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % pure SDS-PAGE|
Target Details NFKB1Product Details anti-NFKB1 Antibody Application Details Handling Images back to top
|Alternative Name||NF-kB p105 / p50 (NFKB1 Antibody Abstract)|
|Background||Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.Synonyms: EBP-1, EBP1, KBF1, NF-kappa-B p50, NFKB1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1|
|Molecular Weight||approx. 105 kDa|
|Pathways||NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction|
Application DetailsProduct Details anti-NFKB1 Antibody Target Details NFKB1 Handling Images back to top
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-NFKB1 Antibody Target Details NFKB1 Application Details Handling back to top