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Human NFKB1 ELISA Kit

Recommended NFKB1 Product (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) ELISA Kits
  • BICDR-1
  • bicdr1
  • ccdc64
  • EBP-1
  • ebp-1
  • KBF1
  • kbf1
  • NF-kappa-B
  • nf-kappa-b
  • nf-kappaB
  • NF-kappaB
  • NF-kappaB1
  • NF-kB
  • NF-KB1
  • NF-kB1
  • NFkappaB
  • NFKB-p50
  • nfkb-p50
  • nfkb-p105
  • NFKB-p105
  • p50
  • p50/p105
  • p105
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
28
18
11
5
4
3
3
3
2
2
1
Application
ELISA
Supplier
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Catalog No. ABIN965406
$ 589.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.001210097 ABIN366568 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.001210097 ABIN578887 Tissue Homogenate, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.001210097 ABIN1380077 Cell ELISA Log in to see
0.001210097 ABIN1380101 Cell ELISA Log in to see
0.001210097 ABIN1380648 Cell ELISA Log in to see
0.001210097 ABIN1380654 Cell ELISA Log in to see
0.001210097 ABIN1380655 Cell ELISA Log in to see
0.001210097 ABIN1381356 Cell ELISA Log in to see
0.001210097 ABIN955956 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.001210097 ABIN1503628 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.001210097 ABIN2866267 Sandwich ELISA Cell Extracts, Tissue Samples Log in to see
0.001210097 ABIN2866280 Sandwich ELISA Cell Extracts, Tissue Samples Log in to see
0.001210097 ABIN2866281 Sandwich ELISA Cell Extracts, Tissue Samples Log in to see
0.001210097 ABIN2113696 Cell ELISA Log in to see
0.001210097 ABIN2113699 Log in to see
0.001210097 ABIN2113694 Cell ELISA Log in to see
0.001210097 ABIN2113697 Cell ELISA Log in to see
0.001210097 ABIN2113695 Cell ELISA Log in to see
0.001210097 ABIN2113698 Log in to see
0.001210097 ABIN2113693 Cell ELISA Log in to see

Similar NFKB1 ELISA Kits

Application / Reactivity Human
ELISA 28 ELISA Kits

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(28), (18), (11), (5), (4), (3), (3), (3), (2), (2), (1)
Application ELISA
Supplier Log in to see

Target details

Product Details NFKB1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name NF kB (p50) (NFKB1 ELISA Kit Abstract)
Background The NF-kB/Rel family of transcription factors is comprised of several structurally related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), c-Rel/NF-kB [1]. Members of this family are responsible for regulating over 150 target genes, including the expression of inflammatory cytokines, chemokines, immunoreceptors and cell adhesion molecules. Because of this, NF-kB has often been called a ‘central mediator of the human immune response' [2]. Acting as dimers, these transcription factors bind to DNA sequences, collectively called kB, sites thereby regulating expression of target genes. In most cells, Rel/ NF-kB transcription complexes are present in an inactive form in the cytoplasm, bound to an inhibitor IB. Certain stimuli result in the phosphorylation, ubiquitination and subsequent degradation of IkB proteins thereby enabling translocation of NF-kB into the nucleus [3]. The most common Rel/NF-kB dimmer in mammals contains p50-RelA (p50/p65) heterodimers and is specifically called NF-kB. One of the target genes activated by NF-kB is that encoding IkBα. This feedback mechanism allows newly-synthesized IkBα to enter the nucleus, remove NF-kB from DNA and transport it back to the cytoplasm thereby restoring its inactive state. The importance of Rel/NF-kB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutics [4-6].
Synonyms: NF-kB Transcription Factor Kit, p50 kit, EIA kit, NF kappaB ELISA Assay
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details NFKB1 ELISA Kit Target details Handling Images back to top
Application Notes NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).
Comment

Detection Kit Type: ELISA Kit

Restrictions For Research Use only

Images

Product Details NFKB1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for NFKB1 ELISA Kit (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN965406) Transcription Factor Binding Assay Schematic
ELISA image for NFKB1 ELISA Kit (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN965406) Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) ELISA Kit (Image 2)
ELISA image for NFKB1 ELISA Kit (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) (ABIN965406) Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) ELISA Kit (Image 3)