anti-Dog (Canine) NFKBIA antibody for Western Blotting

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • LOC100220015
  • LOC100357341
  • Nfkbia
Epitope
Middle Region
71
49
45
39
23
23
22
21
18
18
15
15
14
14
5
5
5
4
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
498
259
214
48
39
28
18
11
2
2
1
1
1
Host
Rabbit
338
165
6
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
19
15
14
12
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
Application
Western Blotting (WB)
386
180
169
89
84
81
66
40
30
19
9
6
5
4
3
3
2
2
1
1
1
1
1
Options
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Catalog No. ABIN2792229
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.347476 ABIN2792116 WB Rabbit N-Term Log in to see Polyclonal 1
3.347476 ABIN252664 IP WB Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 11
3.347476 ABIN252071 ELISA WB Biotin Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413 1
3.347476 ABIN926946 WB Rabbit Middle Region Log in to see Polyclonal
3.347476 ABIN926864 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN470363 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN470399 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1494722 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4324160 IP WB Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324197 IP WB PerCP Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324200 WB DyLight 350 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324205 WB Alexa Fluor 647 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324193 WB DyLight 680 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324201 WB DyLight 405 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324203 WB Alexa Fluor 405 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324196 WB DyLight 755 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324199 WB DyLight 550 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324206 WB Alexa Fluor 700 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324192 WB DyLight 650 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413
1 ABIN4324194 WB DyLight 488 Mouse IgG1 kappa pSer32, pSer36 Log in to see 39A1413

Top referenced anti-NFKBIA antibody for Western Blotting

Similar anti-NFKBIA Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Pig (Porcine) Sheep (Ovine) Dog (Canine) Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies 7 Antibodies 5 Antibodies 3 Antibodies 3 Antibodies 3 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies 2 Antibodies 2 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 377 Antibodies 188 Antibodies 159 Antibodies 44 Antibodies 36 Antibodies 26 Antibodies 17 Antibodies 2 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope Middle Region
(71), (49), (45), (39), (23), (23), (22), (21), (18), (18), (15), (15), (14), (14), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(498), (259), (214), (48), (39), (28), (18), (11), (2), (2), (1), (1), (1)
Host Rabbit
(338), (165), (6)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(19), (15), (14), (12), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Western Blotting (WB)
(386), (180), (169), (89), (84), (81), (66), (40), (30), (19), (9), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792229) Images
Sequence SPYQLTWGRP STRIQQQLGQ LTLENLQMLP ESEDEESYDT ESEFTEFTED
Predicted Reactivity Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against NFKBIA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NFKBIA

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792229) Images back to top
Antigen
Alternative Name NFKBIA (NFKBIA Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine). NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: IKBA, MAD-3, NFKBI
Protein Interaction Partner: BTRC, NEDD9, IKBKB, CHUK, UBC, MTOR, IKBKG, SKP1, FBXW11, UBE2D3, RELA, NPLOC4, VCP, UFD1L, NFKB1, TNF, SUMO1, UBE2D1, COPS3, GPS1, SUMO3, Csnk2b, PRKCA, ST7, STAT1, REL, POLR2C, CD7, ARRB2, ARRB1, SUMO2, PSMA1, ZNF212, IKZF4, ATF4, TBK1, IKBKE, DNAJA3, S
Protein Size: 317
Molecular Weight 35 kDa
Gene ID 4792
NCBI Accession NM_020529, NP_065390
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792229) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 317 AA

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details ProductDetails: References for anti-NFKBIA antibody (ABIN2792229) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-NFKBIA antibody (ABIN2792229)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Truhlar, Mathes, Cervantes, Ghosh, Komives: "Pre-folding IkappaBalpha alters control of NF-kappaB signaling." in: Journal of molecular biology, Vol. 380, Issue 1, pp. 67-82, 2008

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling ProductDetails: References for anti-NFKBIA antibody (ABIN2792229) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (Middle Region) (ABIN2792229) WB Suggested Anti-NFKBIA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: