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anti-Human NFKBIA antibody for Electrophoretic Mobility-Shift Assay

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Reactivity
Human
368
173
137
17
12
8
6
4
2
1
Host
Sheep
320
55
4
1
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
14
11
9
8
6
6
4
3
3
3
3
3
3
3
3
1
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
258
140
119
58
50
49
34
19
12
11
5
5
4
3
2
1
1
1
1
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Catalog No. ABIN121497
$ 511.50
Plus shipping costs $45.00

Similar anti-NFKBIA Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 141 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 60 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 120 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 50 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Reactivity Human
(368), (173), (137), (17), (12), (8), (6), (4), (2), (1)
Host Sheep
(320), (55), (4), (1)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(14), (11), (9), (8), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(258), (140), (119), (58), (50), (49), (34), (19), (12), (11), (5), (5), (4), (3), (2), (1), (1), (1), (1)
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling Images
Specificity This antibody recognises IkB alpha, and identifies a single polypeptide band of 39 kDa in Western blots.
Purification Affinity chromatography
Immunogen Human IkB alpha expressed in E. coli

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling Images back to top
Antigen
Alternative Name NFKBIA / IKBA (NFKBIA Antibody Abstract)
Background Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI
Gene ID 4792
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling Images back to top
Application Notes ELISA: 1:2000. Western Blot: 1:200 - 1:1000. Immunoprecipitation. Gel Super Shift Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide and 0.5 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months