|Application / Reactivity||Human|
|Cell Culture (CC)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||4 Antibodies|
|Dot Blot (DB)||19 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||84 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||28 Antibodies|
|Immunofluorescence (IF)||80 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||40 Antibodies|
|Immunohistochemistry (IHC)||166 Antibodies|
|Antigen||Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies|
|Conjugate||This NFKBIA antibody is un-conjugated Alternatives|
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-NFKBIA AntibodyTarget Details NFKBIA Application Details Handling Images
|Specificity||This antibody recognises IkB alpha, and identifies a single polypeptide band of 39 kDa in Western blots.|
|Immunogen||Human IkB alpha expressed in E. coli|
Target Details NFKBIAProduct Details anti-NFKBIA Antibody Application Details Handling Images back to top
|Alternative Name||NFKBIA / IKBA (NFKBIA Antibody Abstract)|
|Background||Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI|
|Pathways||NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-NFKBIA Antibody Target Details NFKBIA Handling Images back to top
ELISA: 1:2000. Western Blot: 1:200 - 1:1000. Immunoprecipitation. Gel Super Shift Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide and 0.5 % Bovine Serum Albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
|Expiry Date||12 months|