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anti-Human NFKBIA antibody for Intracellular Staining

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha
  • NFKBIA
  • LOC100220015
  • LOC100357341
  • Nfkbia
Epitope
AA 145-302
69
50
44
39
24
24
23
22
19
18
15
15
14
14
5
5
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
496
267
222
49
40
29
17
11
2
2
2
1
1
Host
Mouse
341
160
5
Clonality
Monoclonal
Conjugate
This NFKBIA antibody is conjugated to Alexa Fluor 647
19
15
14
12
8
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
Application
Intracellular Staining (ICS)
385
185
173
84
84
81
66
40
29
20
7
6
5
4
3
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN1176899
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1176898 ICS PE Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 1

Top referenced anti-NFKBIA antibody for Intracellular Staining

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General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope AA 145-302
(69), (50), (44), (39), (24), (24), (23), (22), (19), (18), (15), (15), (14), (14), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(496), (267), (222), (49), (40), (29), (17), (11), (2), (2), (2), (1), (1)
Host Mouse
(341), (160), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIA antibody is conjugated to Alexa Fluor 647
(19), (15), (14), (12), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Intracellular Staining (ICS)
(385), (185), (173), (84), (84), (81), (66), (40), (29), (20), (7), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1176899) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human IkappaBalpha aa. 145-302 Recombinant Protein
Clone 25-IkBa-MAD
Isotype IgG1

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN1176899) Images back to top
Antigen
Alternative Name IkappaBalpha (NFKBIA Antibody Abstract)
Background NF-kappaB is a transcription factor that is a member of the mammalian NF-kappaB/Rel family of proteins. Members of this family are involved in the regulation of cell proliferation, immune function, as well as development. In resting cells, IkappaBalpha binds to and maintains NF-kappaB in the cytoplasm by blocking the nuclear localization sequences of NF-kappaB. In the cellular response to an extracellular signal, IkappaBalpha is phosphorylated and subsequently degraded via the ubiquination-proteasome pathway, allowing NF-kappaB to translocate to the nucleus. Once in the nucleus, NF-kappaB can induce the transcription of IkappaBalpha thereby renewing the cycle so that IkappaBalpha can form a complex with NF-kappaB and maintain it in its cytoplasmic location. IkappaBalpha -/- mice show an increased level of NF-kappaB activity and have been shown to die soon after birth. The 25/IkBa/MAD-3 monoclonal antibody recognizes human IkappaBalpha regardless of phosphorylation status and does not cross-react with mouse IkappaBalpha.
Synonyms: MAD-3, I-kappa-B-alpha, IkB-alpha, NFKBIA, NF-kappa-B inhibitor alpha
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN1176899) Images back to top
Application Notes This Alexa Fluor® 647-conjugated antibody is suitable for intracellular staining of cell lines and primary cells using BD Cytofix™ Fixation Buffer or BD™ Phosflow Lyse/Fix Buffer. Although this antibody can be used with BD™ Phosflow Perm/Wash Buffer I or BD™ Phosflow Perm Buffers II, III or IV, it performs optimally when used with BD™ Phosflow Perm Buffer II.
Sample Volume 5 μL
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN1176899) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-NFKBIA Antibody (ABIN1176899)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Product cited in:

Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, 2000