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anti-Human NFKBIA antibody for Intracellular Staining

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
AA 145-302
51
48
36
29
24
23
22
15
14
13
13
13
7
6
5
5
5
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
413
174
138
17
12
8
6
4
2
1
Host
Mouse
322
97
5
1
Clonality
Monoclonal
Conjugate
This NFKBIA antibody is conjugated to PE
18
13
12
10
6
6
6
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
Application
Intracellular Staining (ICS)
303
179
119
58
54
51
49
34
19
11
6
5
3
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1176898
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008663877 ABIN1176899 ICS Alexa Fluor 647 Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 1
0.0008663877 ABIN2680250 FACS ICS EFluor 660 Mouse IgG2b kappa pSer32, pSer36 Log in to see RILYB3R
0.0008663877 ABIN2680248 FACS ICS PE Rat IgG2b kappa Log in to see MFRDTRK

Top referenced anti-NFKBIA antibody for Intracellular Staining

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General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope AA 145-302
(51), (48), (36), (29), (24), (23), (22), (15), (14), (13), (13), (13), (7), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(413), (174), (138), (17), (12), (8), (6), (4), (2), (1)
Host Mouse
(322), (97), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIA antibody is conjugated to PE
(18), (13), (12), (10), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Intracellular Staining (ICS)
(303), (179), (119), (58), (54), (51), (49), (34), (19), (11), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1176898) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human IkappaBalpha aa. 145-302 Recombinant Protein
Clone 25-IkBa-MAD
Isotype IgG1

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN1176898) Images back to top
Antigen
Alternative Name IkappaBalpha (NFKBIA Antibody Abstract)
Background NF-kappaB is a transcription factor that is a member of the mammalian NF-kappaB/Rel family of proteins. Members of this family are involved in the regulation of cell proliferation, immune function, as well as development. In resting cells, IkappaBalpha binds to and maintains NF-kappaB in the cytoplasm by blocking the nuclear localization sequences of NF-kappaB. In the cellular response to an extracellular signal, IkappaBalpha is phosphorylated and subsequently degraded via the ubiquination-proteasome pathway, allowing NF-kappaB to translocate to the nucleus. Once in the nucleus, NF-kappaB can induce the transcription of IkappaBalpha thereby renewing the cycle so that IkappaBalpha can form a complex with NF-kappaB and maintain it in its cytoplasmic location. IkappaBalpha -/- mice show an increased level of NF-kappaB activity and have been shown to die soon after birth. The 25/IkBa/MAD-3 monoclonal antibody recognizes human IkappaBalpha regardless of phosphorylation status and does not cross-react with mouse IkappaBalpha.
Synonyms: MAD-3, I-kappa-B-alpha, IkB-alpha, NFKBIA, NF-kappa-B inhibitor alpha
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN1176898) Images back to top
Application Notes This Alexa Fluor® 647-conjugated antibody is suitable for intracellular staining of cell lines and primary cells using BD Cytofix™ Fixation Buffer or BD™ Phosflow Lyse/Fix Buffer. Although this antibody can be used with BD™ Phosflow Perm/Wash Buffer I or BD™ Phosflow Perm Buffers II, III or IV, it performs optimally when used with BD™ Phosflow Perm Buffer II.
Sample Volume 5 μL
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN1176898) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References for anti-NFKBIA Antibody (ABIN1176898)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Product cited in:

Nakashio, Fujita, Rokudai, Sato, Tsuruo: "Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis." in: Cancer research, Vol. 60, Issue 18, pp. 5303-9, 2000