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|Application / Reactivity||Human|
|Dot Blot (DB)||19 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||13 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies|
|Immunofluorescence (IF)||61 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies|
|Immunohistochemistry (IHC)||121 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||49 Antibodies|
|Immunoprecipitation (IP)||51 Antibodies|
|Antigen||Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies|
|Conjugate||This NFKBIA antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|1 reference available|
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Product Details anti-NFKBIA AntibodyTarget Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN223053) Images
|Specificity||The antibody detects both phosphorylated and non-phosphorylated forms of IkBα.|
|Immunogen||Recombinant human IkBa|
Target Details NFKBIAProduct Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN223053) Images back to top
|Alternative Name||IkBalpha (NFKBIA Antibody Abstract)|
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/g. External stimuli such TNF-α or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBα.
Synonyms: IkappaBalpha , MAD3, MAD-3, IKBA, NFKBI, IKB-alpha, I-kappa-B-alpha
|Pathways||NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN223053) Images back to top
Western blotting (0.5-4 μg/mL) and Immunoprecipitation (1-4 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: USA
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN223053) Images back to top
|Buffer||100 μg (0.25 mg/mL) immunoaffinity purified mouse monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-NFKBIA Antibody (ABIN223053)Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
|Product cited in:||
Ren, Gao, Wu: "TLR4: the receptor bridging Acanthamoeba challenge and intracellular inflammatory responses in human corneal cell lines." in: Immunology and cell biology, 2010 (PubMed).
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