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anti-Human NFKBIA antibody for Immunoprecipitation

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Reactivity
Human
473
213
176
35
30
22
8
6
2
1
Host
Mouse
331
148
5
1
Clonality
Monoclonal
Conjugate
This NFKBIA antibody is un-conjugated
18
14
13
13
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
362
183
143
86
85
78
73
34
32
19
6
5
4
3
2
1
1
1
1
1
1
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Catalog No. ABIN223053
$ 336.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008946119 ABIN968112 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
0.0008946119 ABIN968113 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
0.0008946119 ABIN967353 IP WB Mouse IgG1 pSer32 Log in to see 39A1413 6
0.0008946119 ABIN967354 IP WB Mouse IgG1 AA 105-291 Log in to see 6A920 6
0.0008946119 ABIN302978 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.0008946119 ABIN303373 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.0008946119 ABIN155061 IP WB Mouse IgG1 Log in to see 6A055
0.0008946119 ABIN208062 FACS IP WB Mouse IgG1, kappa AA 32-291 Log in to see 6A920
0.0008946119 ABIN2707588 IC IF IP IHC WB Rabbit N-Term Log in to see Polyclonal
0.0008946119 ABIN189443 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see E130
0.0008946119 ABIN218609 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0008946119 ABIN241775 IP IHC Mouse IgG1 Log in to see
0.0008946119 ABIN208170 IHC (p) IP ELISA WB Mouse IgG1, kappa pSer32, pSer36 Log in to see 39A1413
0.0008946119 ABIN602026 IHC (fro) IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008946119 ABIN1999497 IP ELISA WB Rabbit IgG AA 2-317 Log in to see 116
0.0008946119 ABIN228557 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008946119 ABIN2469745 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008946119 ABIN1873901 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0008946119 ABIN1999499 IP ELISA WB Rabbit IgG AA 2-317 Log in to see Polyclonal
0.0008946119 ABIN445292 IP WB Rabbit IgG Tyr42 Log in to see EPR2353

Top referenced anti-NFKBIA antibody for Immunoprecipitation

Similar anti-NFKBIA Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 184 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 86 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 34 Antibodies
Immunofluorescence (IF) 80 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 145 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 73 Antibodies
Immunoprecipitation (IP) 86 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Reactivity Human
(473), (213), (176), (35), (30), (22), (8), (6), (2), (1)
Host Mouse
(331), (148), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIA antibody is un-conjugated
(18), (14), (13), (13), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(362), (183), (143), (86), (85), (78), (73), (34), (32), (19), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN223053) Images
Specificity The antibody detects both phosphorylated and non-phosphorylated forms of IkBα.
Purification Affinity purified
Immunogen Recombinant human IkBa
Clone 6A055
Isotype IgG1

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN223053) Images back to top
Antigen
Alternative Name IkBalpha (NFKBIA Antibody Abstract)
Background NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/g. External stimuli such TNF-α or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBα.
Synonyms: IkappaBalpha , MAD3, MAD-3, IKBA, NFKBI, IKB-alpha, I-kappa-B-alpha
Gene ID 4792
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN223053) Images back to top
Application Notes Western blotting (0.5-4 μg/mL) and Immunoprecipitation (1-4 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: USA

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN223053) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer 100 μg (0.25 mg/mL) immunoaffinity purified mouse monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-NFKBIA Antibody (ABIN223053)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Product cited in:

Ren, Gao, Wu: "TLR4: the receptor bridging Acanthamoeba challenge and intracellular inflammatory responses in human corneal cell lines." in: Immunology and cell biology, 2010

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN223053) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (ABIN223053) Western blot analysis of I kappa B alpha in Jurkat cells treated with (right lane) or...