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anti-Mouse (Murine) NFKBIA antibody for Enzyme Immunoassay

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
C-Term
67
53
48
37
23
23
22
22
19
15
14
13
13
13
7
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
473
212
175
35
30
22
8
6
2
1
Host
Rabbit
330
149
5
1
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
18
14
13
13
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
362
183
144
87
85
78
73
34
32
19
5
4
4
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN116524
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088582124 ABIN265483 EIA IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-NFKBIA antibody for Enzyme Immunoassay

Similar anti-NFKBIA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 43 Antibodies 43 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 19 Antibodies 21 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 22 Antibodies 25 Antibodies
Immunofluorescence (IF) 44 Antibodies 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 45 Antibodies
Western Blotting (WB) 132 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope C-Term
(67), (53), (48), (37), (23), (23), (22), (22), (19), (15), (14), (13), (13), (13), (7), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(473), (212), (175), (35), (30), (22), (8), (6), (2), (1)
Host Rabbit
(330), (149), (5), (1)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(18), (14), (13), (13), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(362), (183), (144), (87), (85), (78), (73), (34), (32), (19), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN116524) Images
Specificity This antibody may react non-specifically with other proteins. This antibody is suitable for the detection by immunoblot of Human, Mouse and Rat IκB-alpha. Control Peptide (R1013CP) will compete only with the specific reaction of antiserum with IκB-alpha.
Purification Prepared from monospecific antiserum by Delipidation and Defibrination.
Immunogen IkBa peptide corresponding to a region near the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN116524) Images back to top
Antigen
Alternative Name NFKBIA / IKBA (NFKBIA Antibody Abstract)
Background Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI
Gene ID 4792
NCBI Accession NP_065390
UniProt P25963
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN116524) Images back to top
Application Notes Suitable for Immunoblotting (1/2,000-1/10,000), ELISA (1/20,000-1/100,000) andImmunohistochemistry (1/1,000-1/5,000). Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBa showing a 36 kDa band. Perform all incubations except colordevelopment using TBS supplemented with 0.1 % Tween-20 at room temperature. Block themembrane with 5 % dry milk for 2 h. Add a 1: 1,000 dilution of the primary antibody to themembrane and incubated for 2 h. Perform washes with buffer 4 times for 5' each. Incubatethe immunoblot with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] at1/2,000 for 1 h. Wash with TBS only preceded color development.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN116524) Images back to top
Format Liquid
Concentration 80 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.09 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-NFKBIA Antibody (ABIN116524)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Hoffmann, Levchenko, Scott, Baltimore: "The IkappaB-NF-kappaB signaling module: temporal control and selective gene activation." in: Science (New York, N.Y.), Vol. 298, Issue 5596, pp. 1241-5, 2002

Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996