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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||40 Antibodies||40 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||4 Antibodies|
|Immunochromatography (IC)||1 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||28 Antibodies||33 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies||34 Antibodies|
|Immunohistochemistry (IHC)||69 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||22 Antibodies|
|Immunoprecipitation (IP)||10 Antibodies|
|Western Blotting (WB)||95 Antibodies|
|Antigen||Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This NFKBIA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-NFKBIA AntibodyTarget Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN116524) Images
|Specificity||This antibody may react non-specifically with other proteins. This antibody is suitable for the detection by immunoblot of Human, Mouse and Rat IκB-alpha. Control Peptide (R1013CP) will compete only with the specific reaction of antiserum with IκB-alpha.|
|Purification||Prepared from monospecific antiserum by Delipidation and Defibrination.|
|Immunogen||IkBa peptide corresponding to a region near the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
Target Details NFKBIAProduct Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN116524) Images back to top
|Alternative Name||NFKBIA / IKBA (NFKBIA Antibody Abstract)|
|Background||Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI|
|Pathways||NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN116524) Images back to top
Suitable for Immunoblotting (1/2,000-1/10,000), ELISA (1/20,000-1/100,000) andImmunohistochemistry (1/1,000-1/5,000). Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBa showing a 36 kDa band. Perform all incubations except colordevelopment using TBS supplemented with 0.1 % Tween-20 at room temperature. Block themembrane with 5 % dry milk for 2 h. Add a 1: 1,000 dilution of the primary antibody to themembrane and incubated for 2 h. Perform washes with buffer 4 times for 5' each. Incubatethe immunoblot with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] at1/2,000 for 1 h. Wash with TBS only preceded color development.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN116524) Images back to top
|Concentration||80 mg/mL (by Refractometry)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.09 % (w/v) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-NFKBIA Antibody (ABIN116524)Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Hoffmann, Levchenko, Scott et al.: "The IkappaB-NF-kappaB signaling module: temporal control and selective gene activation." in: Science (New York, N.Y.), Vol. 298, Issue 5596, pp. 1241-5, 2002 (PubMed).
Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996 (PubMed).