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anti-Mouse (Murine) NFKBIA antibody for Flow Cytometry

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
pSer32, pSer36
68
53
49
37
24
23
22
22
19
15
14
13
13
13
7
5
5
5
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
475
214
178
36
31
23
8
6
2
1
Host
Mouse
332
149
5
1
Clonality
Monoclonal
Conjugate
This NFKBIA antibody is conjugated to EFluor 660
19
14
13
13
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
365
184
145
87
84
79
74
34
33
19
6
5
3
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2680250
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2680248 FACS ICS PE Rat IgG2b kappa Log in to see MFRDTRK
0.0010482287 ABIN2664012 FACS Rabbit IgG Log in to see Polyclonal 3
0.0010482287 ABIN4324163 FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324179 FACS ICC IF IHC IHC (p) IP WB DyLight 550 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324180 FACS ICC IF IHC IHC (p) IP WB DyLight 350 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324181 FACS ICC IF IHC IHC (p) IP WB DyLight 405 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324182 FACS ICC IF IHC IHC (p) IP WB DyLight 405LS Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324207 FACS ICC IF IHC IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324208 FACS ICC IF IHC IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324209 FACS ICC IF IHC IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324210 FACS ICC IF IHC IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324171 FACS ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324172 FACS ICC IF IHC IHC (p) IP WB DyLight 650 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324173 FACS ICC IF IHC IHC (p) IP WB DyLight 680 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324174 FACS ICC IF IHC IHC (p) IP WB DyLight 488 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324176 FACS ICC IF IHC IHC (p) IP WB DyLight 755 Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324177 FACS ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324178 FACS ICC IF IHC IHC (p) IP WB PE Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN4324175 FACS ICC IF IHC IHC (p) IP WB HRP Mouse IgG1 kappa AA 32-291 Log in to see 6A920
0.0010482287 ABIN2664011 FACS Rabbit IgG Log in to see Polyclonal

Top referenced anti-NFKBIA antibody for Flow Cytometry

Similar anti-NFKBIA Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 44 Antibodies 184 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 6 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 93 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 49 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 154 Antibodies
Dot Blot (DB) 19 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope pSer32, pSer36
(68), (53), (49), (37), (24), (23), (22), (22), (19), (15), (14), (13), (13), (13), (7), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(475), (214), (178), (36), (31), (23), (8), (6), (2), (1)
Host Mouse
(332), (149), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFKBIA antibody is conjugated to EFluor 660
(19), (14), (13), (13), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(365), (184), (145), (87), (84), (79), (74), (34), (33), (19), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling Images
Specificity This RILYB3R monoclonal antibody recognizes human and mouse nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha (I kappa B alpha) when phosphorylated on serines 32 and 36 (S32/S36). I kappa B alpha is one member of a family of cellular proteins that functions to inhibit classical/canonical NF-kappa B signaling by masking the nuclear localization signals (NLS) of NF-kappa B proteins and keeping them sequestered in an inactive state in the cytoplasm. Classical/canonical NF-kappa B signaling is initiated in response to myriad stimuli including engagement of T cell and B cell receptors, growth factors, and inflammatory stimuli (reactive oxygen species, TNF alpha, IL-1) and results in the activation of the I kappa B kinase (IKK) complex that includes IKK alpha, IKK beta, and NEMO. IKK phosphorylates I kappa B alpha resulting in its ubiquitination, degradation, and subsequent translocation of NF-kappa B transcription factor proteins into the nucleus.
Characteristics This RILYB3R monoclonal antibody recognizes human and mouse nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha (I kappa B alpha) when phosphorylated on serines 32 and 36 (S32/S36). I kappa B alpha is one member of a family of cellular proteins that functions to inhibit classical/canonical NF-kappa B signaling by masking the nuclear localization signals (NLS) of NF-kappa B proteins and keeping them sequestered in an inactive state in the cytoplasm. Classical/canonical NF-kappa B signaling is initiated in response to myriad stimuli including engagement of T cell and B cell receptors, growth factors, and inflammatory stimuli (reactive oxygen species, TNF alpha, IL-1) and results in the activation of the I kappa B kinase (IKK) complex that includes IKK alpha, IKK beta, and NEMO. IKK phosphorylates I kappa B alpha resulting in its ubiquitination, degradation, and subsequent translocation of NF-kappa B transcription factor proteins into the nucleus.
Synonyms: Synonyms: Inhibitor of kappa B alpha
Clone RILYB3R
Isotype IgG2b kappa

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling Images back to top
Antigen
Alternative Name IkBa (NFKBIA Antibody Abstract)
Research Area Transcription Factors
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling Images back to top
Application Notes Applications reported: This RILYB3R antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This RILYB3R antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: Protocol A and Protocol C are recommended for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. Page 2 of 2 Anti-Human/Mouse phospho-IkBa (S32/S36) eFluor® 660
Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pSer32) (EFluor 660) (ABIN2680250) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (pSer32), (pSer36) antibody (EFluor 660)