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anti-Mouse (Murine) NFKBIB antibody for Enzyme Immunoassay

Recommended NFKBIB Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, beta (NFKBIB) Antibodies
  • IKappaBbeta
  • IkB
  • IKB-beta
  • IKBB
  • IkBb
  • NFKBIB
  • TRIP9
Epitope
C-Term
34
27
17
15
12
7
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
159
64
54
4
4
Host
Rabbit
131
32
Clonality
Polyclonal
Conjugate
This NFKBIB antibody is un-conjugated
6
6
5
3
3
3
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
108
64
64
29
24
18
11
7
3
2
1
1
Supplier
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Catalog No. ABIN116525
$ 500.50
Plus shipping costs $45.00

Top referenced anti-NFKBIB antibody for Enzyme Immunoassay

Similar anti-NFKBIB Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 10 Antibodies 13 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 4 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 16 Antibodies 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 13 Antibodies
Immunoprecipitation (IP) 4 Antibodies 4 Antibodies
Western Blotting (WB) 29 Antibodies 37 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, beta (NFKBIB) Antibodies
Epitope C-Term
(34), (27), (17), (15), (12), (7), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(159), (64), (54), (4), (4)
Host Rabbit
(131), (32)
Clonality
Polyclonal
Conjugate This NFKBIB antibody is un-conjugated
(6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(108), (64), (64), (29), (24), (18), (11), (7), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIB Antibody

Target Details NFKBIB Application Details Handling References for anti-NFKBIB Antibody (ABIN116525) Images
Specificity Recognition is at the highly conserved C-terminal region. Anti-IκBβ may react non-specifically with other proteins. Control peptide (R1014CP) will compete only with the specific reaction of antiserum with IκBβ.
Purification Prepared from monospecific antiserum by delipidation and defibrination
Immunogen IkB beta peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Target Details NFKBIB

Product Details anti-NFKBIB Antibody Application Details Handling References for anti-NFKBIB Antibody (ABIN116525) Images back to top
Antigen
Alternative Name NFKBIB / IKBB (NFKBIB Antibody Abstract)
Background NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.Synonyms: I-kappa-B-beta, IkB-B, IkB-beta, IkappaBbeta, NF-kappa-B inhibitor beta, TR-interacting protein 9, TRIP9, Thyroid receptor-interacting protein 9
Gene ID 4793
NCBI Accession NP_001001716
UniProt Q15653
Pathways NF-kappaB Signaling

Application Details

Product Details anti-NFKBIB Antibody Target Details NFKBIB Handling References for anti-NFKBIB Antibody (ABIN116525) Images back to top
Application Notes Suitable for Immunoprecipitation, Western blotting (171,000, shows a 45 kD band), ELISAand Dot Blot. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBβ at a dilution of 1: 1000 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-IκBβ is suitable for thedetection by immunoblot of human, mouse and rat IκBβ.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFKBIB Antibody Target Details NFKBIB Application Details References for anti-NFKBIB Antibody (ABIN116525) Images back to top
Format Liquid
Concentration 90 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

References for anti-NFKBIB Antibody (ABIN116525)

Product Details anti-NFKBIB Antibody Target Details NFKBIB Application Details Handling Images back to top
Background publications

Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996