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anti-Human NFKBIE antibody for Immunocytochemistry

Recommended NFKBIE Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE) Antibodies
  • NFKBIE
  • IKBE
  • Slc35b2
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
  • nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon
  • NFKBIE
  • Nfkbie
Epitope
AA 207-440
36
13
13
4
3
3
2
2
2
2
2
2
2
2
1
1
1
Reactivity
Human
99
57
38
6
1
Host
Rabbit
100
2
Clonality
Polyclonal
Conjugate
This NFKBIE antibody is un-conjugated
4
4
3
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
63
40
33
18
15
11
4
2
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Available images

Catalog No. ABIN1859320
$ 385.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4324222 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN2883910 ICC IHC ELISA WB Rabbit IgG pSer22 Log in to see Polyclonal
1 ABIN1844185 ICC IF IHC WB Rabbit pSer22 Log in to see Polyclonal
1 ABIN2972613 ICC IF WB Rabbit Center Log in to see Polyclonal

Similar anti-NFKBIE Antibodies

Application / Reactivity Human
ELISA 19 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Western Blotting (WB) 62 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE) Antibodies
Epitope AA 207-440
(36), (13), (13), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(99), (57), (38), (6), (1)
Host Rabbit
(100), (2)
Clonality
Polyclonal
Conjugate This NFKBIE antibody is un-conjugated
(4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(63), (40), (33), (18), (15), (11), (4), (2)
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Product Details anti-NFKBIE Antibody

Target Details NFKBIE Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH VGALSPQQLE ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR PEPGRGTSHS LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE GTSGKTALHL AVETQERGLV QFLLQAGAQV DARMLNGCTP
Specificity It has been selected for its ability to recognize IkBe in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen IkBe (AA 207-440)
Isotype IgG

Target Details NFKBIE

Product Details anti-NFKBIE Antibody Application Details Handling Images back to top
Antigen
Pathways NF-kappaB Signaling

Application Details

Product Details anti-NFKBIE Antibody Target Details NFKBIE Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant IkBe (Arg207~Pro440) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions For Research Use only

Handling

Product Details anti-NFKBIE Antibody Target Details NFKBIE Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-NFKBIE Antibody Target Details NFKBIE Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIE antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon) (AA 207-440) (ABIN1859320) anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE) (AA 207-440) antibody